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Effect of salts and surfactants on the partitioning of Fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch

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Bioseparation

Abstract

An aqueous two-phase system composed by a thermoseparating random copolymer of ethylene oxide/propylene oxide 50/50 (%w/w), Breox, and hydroxypropyl starch – Reppal PES 100 was evaluated for the partitioning of Fusarium solani pisi recombinant cutinase. The effect of several additives on the partitioning of pure cutinase was evaluated. Micelles of sodium dodecanoate provided a ten-fold increase of the partitioning coefficient (K=9) and recovery yields of 60-75%. The phase diagrams of the systems composed of Breox, Reppal and sodium dodecanoate were determined and it was found that in systems with high surfactant concentrations, the binodal was moved to lower polymer concentrations, enabling a two-phase system with 6% (w/w) of each polymer.

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Authors and Affiliations

  1. Centro de Engenharia Biológica e Química, Instituto Superior Técnico, 1049-001, Lisboa, Portugal

    Maria Teresa Cunha, Joaquim M.S. Cabral & Maria Raquel Aires-Barros

  2. Department of Biochemistry, University of Lund, P.O. Box 124, S-221 00, Lund, Sweden

    Maria Teresa Cunha & Folke Tjerneld

Authors
  1. Maria Teresa Cunha
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  2. Joaquim M.S. Cabral
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  3. Folke Tjerneld
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  4. Maria Raquel Aires-Barros
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Teresa Cunha, M., Cabral, J.M., Tjerneld, F. et al. Effect of salts and surfactants on the partitioning of Fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch. Bioseparation 9, 203–209 (2000). https://doi.org/10.1023/A:1008132108117

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