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Purification of mouse monoclonal anti-HCG IgG1 from ascites fluid by hydroxylapatite chromatography

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Biotechnology Techniques

Summary

Mouse monoclonal IgG1 antibody to human chorionic gonadotropin from ascites fluid was purified by one-step hydroxylapatite chromatography. The purified monoclonal antibody is over 90% yield and essentially free of contaminating mouse IgG found in ascites fluid.

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Authors and Affiliations

  1. Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Chongyang, P.O.Box 150, 131, Seoul, Korea

    Hack-Joo Kim & Si Myung Byun

  2. Research Laboratories Dong-A Pharmaceutical Co., 131, Seoul, Korea

    Gye Won Kim, Sang Hoon Cha & Won Bae Kim

Authors
  1. Hack-Joo Kim
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  2. Gye Won Kim
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  3. Sang Hoon Cha
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  4. Won Bae Kim
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  5. Si Myung Byun
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Kim, HJ., Kim, G.W., Cha, S.H. et al. Purification of mouse monoclonal anti-HCG IgG1 from ascites fluid by hydroxylapatite chromatography. Biotechnol Tech 1, 269–274 (1987). https://doi.org/10.1007/BF00155467

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  • DOI: https://doi.org/10.1007/BF00155467

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