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Immunoaffinity purification of monoclonal antibodies: In search of an elution buffer of general applicability

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Biotechnology Techniques

Summary

Of a number of elution buffers tested, 4MgCl2 in 25% ethylene glycol was the most eficient for recovering active monoclonal antibodies from immunoaffinity columns. This solution may be useful as a general purpose eluant for monoclonal antibody purification.

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References

  • Boyd, S. and Yamazaki, H. (1993). Biotech. Letters, 7; 827–832.

    Google Scholar 

  • Civalleri, L., Federico, R. and Pini, C. (1986). Biotech. Appl. Biochem., 8; 387–392.

    Google Scholar 

  • Cochet, S., Hasnaoui, M., Debbia, A., Kroviarski, Y., Lambin, P., Cartron, J. and Bertrand, O. (1994). J.Chromatoq., 663; 175–186.

    Google Scholar 

  • Firer, MA., Ben-Zion, Z., Kostikov, M. and Irlin, Y. (1995). Isr. J. Med. Sci., 31;382–388.

    Google Scholar 

  • Harlow, E. and Lane, D. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory (Publ.,) 1988. Chp.13.

  • Johnstone, A. and Thorpe, R. (1990). Immunochemistry in Practice. Chp.10. 2nd. Ed., Blackwell Scientific Publications

  • Tsang, VCW. and Wilkins, PP. (1991). J. Immunol. Methods, 138; 291–299.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Immunology and Cell Biology, Research Institute, Academic College of Judea and Samaria, P.O. Box 3, 44837, Ariel, Israel

    Ben-David A. & Firer M. A.

Authors
  1. Ben-David A.
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  2. Firer M. A.
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Ben-David, A., Firer, M.A. Immunoaffinity purification of monoclonal antibodies: In search of an elution buffer of general applicability. Biotechnol Tech 10, 799–802 (1996). https://doi.org/10.1007/BF00222569

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  • DOI: https://doi.org/10.1007/BF00222569

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