Abstract
Lipase A and B from Geotrichum candidum CMICC 335426 were functionally expressed and secreted in Pichia pastoris. Lipase B exhibits a unique substrate specificity for long-chain cis-δ9 unsaturated triacylglycerols. Thus, using different cultivation conditions, the production of recombinant lipase B was optimised, yielding 200 000 Units per liter culture. After overexpression lipase B was purified by diafiltration (372 U/mg). The investigated enzyme properties were comparable to those reported for the native enzyme.
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Catoni, E., Schmidt-Dannert, C., Brocca, S. et al. Overexpression of lipase A and B of Geotrichum candidum in Pichia pastoris: High-level production and some properties of functional expressed lipase B. Biotechnology Techniques 11, 689–695 (1997). https://doi.org/10.1023/A:1018419729009
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DOI: https://doi.org/10.1023/A:1018419729009