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Pulsed column reactor as a novel tool for continuous enzymatic synthesis of peptide in an aqueous/organic biphasic medium

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Biotechnology Techniques

Abstract

We propose a novel effective method for a continuous peptide synthesis in an aqueous/organic biphasic medium using a pulsed column reactor. N-Formyl-l-aspartyl-l-phenylalanine methyl ester was enzymatically synthesized continuously. With this extractive method using a pulsed column reactor, we can synthesize peptides with a stable performance even if a peptide (or a peptide-amino acid complex) is precipitated due to its high hydrophobicity.

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Authors and Affiliations

  1. Department of Chemical Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo, 169-8555, Japan

    Yoshihiko Murakami, Shigemitsu Hayashi, Akinori Takehara & Akira Hirata

Authors
  1. Yoshihiko Murakami
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  2. Shigemitsu Hayashi
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  3. Akinori Takehara
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  4. Akira Hirata
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Murakami, Y., Hayashi, S., Takehara, A. et al. Pulsed column reactor as a novel tool for continuous enzymatic synthesis of peptide in an aqueous/organic biphasic medium. Biotechnology Techniques 13, 165–168 (1999). https://doi.org/10.1023/A:1008954129945

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  • DOI: https://doi.org/10.1023/A:1008954129945

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