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Complex between α-Chymotrypsin and poly(ethylene glycol) catalytically active in organic media

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Biotechnology Techniques

Abstract

α-Chymotrypsin and poly(ethylene glycol) were mixed in an aqueous phosphate buffer solution and lyophilized. The preparation was readily soluble in anhydrous isooctane. The polymer/enzyme complex, i.e., poly(ethylene glycol)/α-chymotrypsin, was catalytically active in anhydrous isooctane even when the molecular ratio of them was unity.

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Authors and Affiliations

  1. Department of Chemical Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo, 169-8555, Japan

    Yoshihiko Murakami & Akira Hirata

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  1. Yoshihiko Murakami
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  2. Akira Hirata
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Murakami, Y., Hirata, A. Complex between α-Chymotrypsin and poly(ethylene glycol) catalytically active in organic media. Biotechnology Techniques 13, 545–548 (1999). https://doi.org/10.1023/A:1008987431361

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  • DOI: https://doi.org/10.1023/A:1008987431361

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