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Intracellular pH in singleSaccharomyces cerevisiae cells

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Biotechnology Techniques

Abstract

Saccharomyces cerevisiae cells can be stained with the pH dependent fluorochrome BCECF, to monitor shifts in intracellular pH in individual cells using flow cytometry. Cells stained in the presence of ethanol were found to be stained as much as 100 times more intensely as cells without ethanol. Cells that have been starved for the carbon source showed a significant shift in intracellular pH upon refeeding with a metabolizable carbon source within 3 minutes.

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Authors and Affiliations

  1. Institute for Advanced Studies in Biological Process Technology and Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, USA

    B. J. Hernlem & F. Srienc

  2. Institute for Advanced Studies in Biological Process Technology and Department of Chemical Engineering and Materials Science, University of Minnesota, St. Paul, MN, USA

    B. J. Hernlem & F. Srienc

Authors
  1. B. J. Hernlem
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  2. F. Srienc
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Hernlem, B.J., Srienc, F. Intracellular pH in singleSaccharomyces cerevisiae cells. Biotechnol Tech 3, 79–84 (1989). https://doi.org/10.1007/BF01875557

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  • DOI: https://doi.org/10.1007/BF01875557

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