Skip to main content
SpringerLink
Log in

Electrophoretic desorption of concanavalin a from an affinity matrix: A rapid method for the preparation of carbohydrate free concanavalin A

  • Published:
Biotechnology Techniques

Summary

Electrophoretic desorption of concanavalin A from Sephadex G 50 is described. The method is simple, rapid, eliminates dialysis and biologically active carbohydrate free lectin is obtained within an hour.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Agrawal, B.B.L. and Goldstein, I.J. (1972) In:Methods in Enzymology, V. Ginsburg, ed. Vol. 28, pp. 313–316, New York: Academic Press.

    Google Scholar 

  • Bailon, P. and Weber, D.V. (1988)Nature 335: 839–840.

    Google Scholar 

  • Burton, S.J. (1987) Affinity chromatography, In:Techniques in Molecular Biology, J.M. Walker and W. Gaastra, eds. Vol. 2, pp.55–81, New York: Macmillan Publishing Company.

    Google Scholar 

  • Morgan, M.R.A., Slater, A.N. and Dean, P.D.G. (1979)Anal. Biochem., 92: 144–146.

    Google Scholar 

  • Surolia, A. Prakash, N., Bishayee, S. and Bacchawat, B.K. (1973)Indian J. Biochem. Biophys., 10: 145–148.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Zoology, University of Poona, 411 007, Pune, India

    George Varghese & A. M. Diwan

Authors
  1. George Varghese
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. M. Diwan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Varghese, G., Diwan, A.M. Electrophoretic desorption of concanavalin a from an affinity matrix: A rapid method for the preparation of carbohydrate free concanavalin A. Biotechnol Tech 3, 233–234 (1989). https://doi.org/10.1007/BF01876054

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01876054

Keywords

Search

Navigation