Skip to main content
SpringerLink
Log in

A study of nicotine release from tobacco hairy roots by transient technique

  • Published:
Biotechnology Techniques

Abstract

A material balancing method for determining the productivity of nicotine-releasing Nicotiana tabacum hairy roots is presented. Diffusive nicotine release from the intracellular environment of N. tabacum to its medium is based upon an equilibrium partitioning process. By removing nicotine from a culture's medium, subsequent transient release from the tissue is induced, as is the heightened production of nicotine. Under normal conditions, sixteen hours is required for releasing tissue to re-establish equilibrium with the medium. 100 ppm of the surfactant Triton X-100 was used to enhance the rate of nicotine release and to reduce the return to equilibrium to four hours without toxic side-effects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baldwin, I.T. (1988). Journal of Chemical Ecology 14 (4), 1113–1120.

    Google Scholar 

  • Brodelius, P., and Nilsson, K. (1983). Applied Microbiology and Biotechnology 17, 275–280.

    Google Scholar 

  • Charlwood, B.V. (1990). In Secondary Products from Plant Tissue Culture; Charlwood, B.V., and Rhodes, M.J.C., Eds.; Oxford University Press; New York; pp 1–21.

    Google Scholar 

  • Gamborg, O.L., Miller, R.A., and Ojima, K. (1968). Experimental Cell Research 50, 151–158.

    Google Scholar 

  • Parr, A.J., Robins, R.J., and Rhodes, M.J.C. (1986). Physiologie Vegetale 24 (4), 419–429.

    Google Scholar 

  • Parr, A.J., and Hamill, J.D. (1987). Phytochemistry 26 (12), 3241–3245.

    Google Scholar 

  • Renaudin, J.P., and Guern, J. (1982). Physiologie Vegetale 20 (3), 533–547.

    Google Scholar 

  • Rhodes, M.J.C., Hilton, M., Parr, A.J., Hamill, J.D., and Robins, R.J. (1986). Biotechnology Letters 8 (6), 415–420.

    Google Scholar 

  • Saunders, J.A., and Blume, D.E. (1981). Journal of Chromatography 205, 147–154.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. BioProcessing Institute, Department of Chemical Engineering, Lehigh University, 18015, Bethlehem, PA, U.S.A.

    William A. Larsen & James T. Hsu

  2. Biotechnology Institute, Pennsylvania State University, 16802, University Park, PA, U.S.A.

    Hector E. Flores & Arthur E. Humphrey

Authors
  1. William A. Larsen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. James T. Hsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hector E. Flores
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Arthur E. Humphrey
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Larsen, W.A., Hsu, J.T., Flores, H.E. et al. A study of nicotine release from tobacco hairy roots by transient technique. Biotechnol Tech 7, 557–562 (1993). https://doi.org/10.1007/BF00156328

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00156328

Keywords

Search

Navigation