Abstract
A 60-Mb murine chromosome consisting of murine pericentric satellite DNA and two bands of integrated marker and reporter genes has been generated de novo in a rodent/human hybrid cell line (mM2C1). This prototype mammalian artificial chromosome platform carries a normal centromere, and the expression of its β-galactosidase reporter gene has remained stable under selection for over 25 months. The novel chromosome was transferred by a modified microcell fusion method to mouse [L-M(TK−)], bovine (P46) and human (EJ30) cell lines. In all cases, the chromosome remained structurally and functionally intact under selection for periods exceeding 3 months from the time of transfer into the new host. In addition, the chromosome was retained in three first- generation tumours when L-M(TK−) cells containing the chromosome were xenografted in severe combined immunodeficiency mice. These data support that a murine satellite DNA-based artificial chromosome can be used as a functional mammalian artificial chromosome and can be maintained in vivo and in cells of heterologous species in vitro.
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Telenius, H., Szeles, A., Keresö, J. et al. Stability of a Functional Murine Satellite DNA-based Artificial Chromosome Across Mammalian Species. Chromosome Res 7, 3–7 (1999). https://doi.org/10.1023/A:1009215026001
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DOI: https://doi.org/10.1023/A:1009215026001