Skip to main content
Log in

Production of multidomain complement glycoproteins in insect cells

  • Published:
Cytotechnology Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Abbreviations

AcMNPV:

Autographa californica nuclear polyhedrosis virus

C1:

first component of complement

C1q, C1r, C1s:

subcomponents of C1

CCP:

complement control protein

EGF:

epidermal growth factor

High5:

Trichoplusia ni cell line

SCR:

short concensus repeat

Sf9:

Spodoptera frugiperda 9 cell line

References

  • ArlaudGJ, ColombMG & GagnonJ (1987) A Functional model of the human C1 complex. Immunol. Today 8: 106–111.

    Google Scholar 

  • BockSC, SkriverK, NielsenE, ThogersenHC, WimanB, DonaldsonVH, EddyRL, MarrimanJ, RadziejewskaE, HuberR, ShawsTB & MagnussonS (1986) Human C1 inhibitor: primary structure, cDNA cloning, and chromosomal localization. Biochemistry 25: 4292–4301.

    Google Scholar 

  • BorsosT, LoosM, ChapuisRM, MedicusR & IslikerH (1980) A novel way of relating the structure of C1q to the hemolytic activity of the first component of complement. Mol. Immunol. 17: 1415–1421.

    Google Scholar 

  • CoutinhoM, AulakKS & DavisAE (1994) Functional analysis of the serpin domain of C1-inhibitor. J. Immunol. 154: 3648–3654.

    Google Scholar 

  • DavidsonDJ & CastellinoFJ (1991) Asparagine-linked oligosaccharide processing in Lepidopteran insect cells. Temporal dependence of the nature of the ologosaccharides assembled on Asparagine-289 of recombinant human plasminogen produced in baculovirus vector infected Spodoptera frugiperda (IPLB-SF-21AE) cells. Biochemistry 30: 6167–6174.

    Google Scholar 

  • Dobó J (1993) Diploma thesis. Institute of Enzymology, Budapest.

  • DonaldsonVH & EvansRR (1963) A biochemical abnormality in hereditary angioneurotic edema: absence of serum inhibitor of C1-esterase. Am. J. Med. 35: 37–44.

    Google Scholar 

  • ElderingE, NuijensJH & HackC (1988) Expression of functional human C1 inhibitor in COS cells. J. Biol. Chem. 263: 11776–11779.

    Google Scholar 

  • GálP, SárváriM, SzilágyiK, ZávodszkyP & SchumakerVN (1989) Expression of hemolytically active human complement component C1r proenzyme in insect cells using a baculovirus vector. Complement and Inflammation. 6: 433–441.

    Google Scholar 

  • GálP, CsehS, SchumakerVN & ZádvodszkyP (1994) The structure and function of the first component of complement: genetic engineering approach. Acta Microbiol. Immunol. Hung. 41: 361–380.

    Google Scholar 

  • Hajós J (1993) Diploma thesis. Institute of Enzymology, Budapest.

  • KnobelHR, VilligerW & IslikerH (1975) Chemical analysis and electron microscopy studies of human C1q prepared by different methods. Eur. J. Immunol. 5: 78–82.

    Google Scholar 

  • KurodaK, GeyerH, GeyerR, DoerflerW & KlenkHD (1990) The oligosaccharides of influenza virus hemagglutinin expressed in insect cells by a baculovirus vector. Virology 174: 418–429.

    Google Scholar 

  • LeytusSP, KurachiK, SakariassenS & DavieEW (1986) Nucleotide sequence of cDNA coding for human complement C1r. Biochemistry 25: 4855–4863.

    Google Scholar 

  • LuoC, ThielensNM, GagnonJ, GálP, SárváriM, TsengY, TosiM, ZávodszkyP, ArlaudGJ & SchumakerVN (1992) Recombinant human complement subcomponent C1s lacking β-hydroxyasparagine, sialic acid, and one of its two carbohydrate chain still reassembles with C1q and C1r to form a functional complex. Biochemistry 31: 4254–4262.

    Google Scholar 

  • MackinnonCM, CarterPE, SmythSJ, DunbarB & FothergillJE (1987) Molecular cloning of cDNA for human complement component C1s. The complete amino acid sequence. Eur. J. Biochem. 169: 547–553.

    Google Scholar 

  • MonkovicDD, VanDusenVJ, PetroskiCJ, GarskiVM, SardanaMK, ZávodszkyP, SternAM & FriedmanPA (1992) Invertebrate aspartyl/asparaginyl β-hydroxylase: potential modification of endogenous epidermal growth factor-like modules. Biochem. Biophys. Res. Comm. 189: 233–241.

    Google Scholar 

  • PatthyL (1994) Modular design of proteases of coagulation, fibrinolysis, and complement activation: implications for protein engineering and structure-function studies. In: LorandL & MannKG (ed.) Methods in Enzymology. Vol. 222 (pp. 10–22) Academic Press, New York.

    Google Scholar 

  • PetryF, ReidKBM & LoosM (1989) Molecular cloning and characterization of the complementary DNA coding for the B-chain of murine C1q. FEBS Lett. 258: 89–93.

    Google Scholar 

  • PetryF, ReidKBM & LoosM (1991) Gene expression of the A- and B-chain of mouse C1q in different tissue and the characterization of the recombinant A-chain. J. Immunol. 147: 3988–3993.

    Google Scholar 

  • PetryF, ReidKBM & LoosM (1992) Isolation, sequence analysis and characterization of cDNA clones coding for the C chain of mouse C1q. Eur. J. Biochem. 209: 129–134.

    Google Scholar 

  • PrzysieckiCT, StaggersJR, RamjitHG, MussanDG, SternAM, BennettCD & FriedmanPA (1987) Occurrence of β-hydroxylated asparagine residues in non-vitamin K dependent proteins containing epidermal growth factor-like domains. Proc. Natl. Acad. Sci. 84: 7856–7860.

    Google Scholar 

  • RappHJ & BorsosT (1976) Molecular basis of complement action. Meredith Corporation, New York.

    Google Scholar 

  • ReidKBM & PorterRR (1976) Subunit composition and structure of subcomponent C1q of the first component of human complement. Biochem. J. 155: 19–23.

    Google Scholar 

  • SárváriM, CsikósGy, SassM, GálP, SchumakerVN & ZávodszkyP (1990) Ecdysteroids increase the yield of recombinant protein produced in baculovirus insect cell expression system. Biochem. Biophys. Res. Comm. 167: 1154–1161.

    Google Scholar 

  • SchumakerVN, ZávodszkyP & PoonP (1987) Activation of the first component of complement. Ann. Rev. Immunol. 5: 21–42.

    Google Scholar 

  • SchumakerVN, TsengY, PoonPH, BianchinoAC & ZávodszkyP (1989) Spontaneous activation of reconstituted and serum C1 and the role of C1-inhibitor. Behring Institute Mitteilungen 84: 102–110.

    Google Scholar 

  • SellarGC, BlakeDJ & ReidKBM (1991) Characterization and organization of the genes encoding the A-, B- and C chains of human complement subcomponent C1q. Biochem. J. 274: 481–490.

    Google Scholar 

  • StenfloJ, LundwallA & DahlbackB (1987) β-Hydroxyasparagine in domains homologous to the epidermal growth factor precursor in vitamin K-dependent proteins. Proc. Natl. Acad. Sci. 84: 368–372.

    Google Scholar 

  • Summers MD & Smith GE (1987) A manual of methods for baculovirus vectors and insect cell culture procedures. Tex. Agric. Exp. Stn., Bull., 1555, College Station, Texas.

  • ThimL, BjoernS, ChristensenM, NicolaisenEM, Lund-HansenT, PedersenAH & HednerU (1988) Amino acid sequence and posttranslational modifications of human factor VIIa from plasma and transfected baby hamster kidney cells. Biochemistry 27: 7785–7793.

    Google Scholar 

  • TosiM, DuponchelC, MeoT & JulieC (1987) Complete cDNA sequence of human complement C1s and close physical linkage of the homologous genes C1s and C1r. Biochemistry 26: 8516–8524.

    Google Scholar 

  • ThielensNVM, VanDorsselaerA, GagnonJ & ArlaudGJ (1990) Chemical and functional characterization of fragment of C1s containing the epidermal growth factor homology region. Biochemistry 14: 3570–3578.

    Google Scholar 

  • TschoppJ, VilligerW, FuchsH, KilcherrE & EngelJ (1980) Assembly of subcomponents C1r and C1s of the first component of complement: electron microscope and ultracentrifugal studies. Proc. Narl. Acas. Sci. 77: 7014–7019.

    Google Scholar 

  • TsengY, PoonPH, ZávodszkyP & SchumakerVN (1991) Spontaneous activation of serum C1 in vitro. Role of C1 inhibitor. J. Immunol. 147: 1884–1890.

    Google Scholar 

  • VlakJM, SchlaegerE-J & BernardAR (1992) Workshop on baculovirus and recombinant protein production processes. Editiones Roche, Basel.

    Google Scholar 

  • ZávodszkyP, GálP, CsehS & SchumakerVN (1993) Protein engineering studies on C1r and C1s. Behring Inst. Res. Comm. 93: 103–114.

    Google Scholar 

  • ZiccardiRJ (1982) Spontaneous activation of the first component of human complement (C1) by an intramolecular catalytic activity. J. Immunol. 128: 2500–2504.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Závodzky, P., Cseh, S. Production of multidomain complement glycoproteins in insect cells. Cytotechnology 20, 279–288 (1996). https://doi.org/10.1007/BF00350407

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00350407

Key words

Navigation