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Investigation into chromosomal stability of in-vitro growing cells from a transformed monosomic addition plant of beet

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Summary

Crown galls were obtained from a monosomic addition plant of beet, carrying an extra chromosome of Beta procumbens, after transformation with a wild-type Agrobacterium tumefaciens strain. Analysis of peroxidase and esterase patterns obtained after cellulose acetate gel electrophoresis and iso-electric focusing respectively, gave evidence that no preferential loss of the alien chromosome on repeated subculture in-vitro from the crown galls occurred.

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Huizing, H.J., van der Molen, W. & Krens, F.A. Investigation into chromosomal stability of in-vitro growing cells from a transformed monosomic addition plant of beet. Euphytica 39 (Suppl 3), 177–184 (1988). https://doi.org/10.1007/BF00043381

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