Summary
Leaf explants of apple cvs Gala and Golden Delicious were infected with the Agrobacterium tumefaciens strain AGL0(pMOG410). The effects of a 2 d preculture of the explants before infection and the addition of 5-azacytidine to the selection medium were studied. The percentages of GUS-positive explants after 5 w did not significantly alter due to these treatments. One of the ‘Gala’ shoots, which was removed from a leaf explant cultured for 8 w on selection medium, proved to be GUS-positive and will be analyzed further. In general, however, it should be concluded that regeneration of transgenic shoots directly from leaf tissue was not very effective.
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Abbreviations
- MS:
-
Murashige and Skoog medium
- GD:
-
‘Golden Delicious’
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Schaart, J.G., Puite, K.J., Kolova, L. et al. Some methodological aspects of apple transformation by Agrobacterium . Euphytica 85, 131–134 (1995). https://doi.org/10.1007/BF00023941
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DOI: https://doi.org/10.1007/BF00023941