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A simple method for the determination of the specific activity of125I-tracer used in radioimmunoassay

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Abstract

Knowledge of the specific activity of the125I-tracer is essential for optimization and for calculation of RIA parameters. The specific activity of the125I-thyroxin used in thyroxin radioimmunoassay /RIA/ has been determined by a simple method involving combination of RIA and displacement analysis. It has been compared with the value obtained by the conventional method based on radioiodination data. Our studies indicate that even for a non-protein hormone like thyroxin the specific activity of125I-thyroxin derived from iodination data is not reliable. The specific activities obtained by displacement analysis were consistent with the experimental findings.

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Authors and Affiliations

  1. Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Trombay, 400 085, Bombay, India

    R. S. Mani

  2. Regional Centre for Radiopharmaceuticals, Bhabha Atomic Research Centre, KMIO Campus, 560 029, Bangalore, India

    V. Bhupal

Authors
  1. V. Bhupal
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  2. R. S. Mani
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Bhupal, V., Mani, R.S. A simple method for the determination of the specific activity of125I-tracer used in radioimmunoassay. Journal of Radioanalytical and Nuclear Chemistry Letters 107, 377–383 (1986). https://doi.org/10.1007/BF02164203

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  • DOI: https://doi.org/10.1007/BF02164203

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