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Reversible, Thermally Induced Domain Unfolding in Oligomeric Proteins. Spectral and DSC measurements

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Abstract

Spectral and differential scanning calorimetry (DSC) results for three oligomeric proteins are briefly reviewed. (A) Reversible, thermally-induced partial unfolding reactions in dodecameric glutamine synthetase from E. coli involve cooperative, two two-state transitions of subunits and demonstrate communication among subunits. (B) Thermal unfolding of intact Acanthamoeba myosin II is more cooperative than that of mammalian skeletal muscle myosin. Nucleotide-induced conformational changes thermally stabilize head domains in both myosins. The long dimeric coiled-coil rod of Acanthamoeba myosin II undergoes a reversible, cooperative, single two-state thermal transition with concomitant chain dissociation. (C) The amino terminal domain of enzyme I of the E. coliPEP:sugar phosphotransferase system is destabilized by phosphorylation of the active-site His 189.

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References

  1. E. R. Stadtman and A. Ginsburg, Enzymes (3rd Ed.), 10 (1974) 755.

    CAS  Google Scholar 

  2. S. G. Rhee, P. B. Chock and E. R. Stadtman, Adv. Enzymol., 62 (1989) 37.

    CAS  Google Scholar 

  3. A. Ginsburg, Adv. Protein Chem., 26 (1972) 1.

    CAS  Google Scholar 

  4. G. Colombo and J. J. Villafranca, J. Biol. Chem., 261 (1986) 10587.

    CAS  Google Scholar 

  5. R. C. Valentine, B. M. Shapiro and E. R. Stadtman, Biochemistry, 7 (1968) 2143.

    Article  CAS  Google Scholar 

  6. R. J. Almassy, C. A. Janson, R. Hamlin, N. H. Xuong and D. Eisenberg, Nature, 323 (1986) 304.

    Article  CAS  Google Scholar 

  7. M. M. Yamashita, R. J. Almassy, C. A. Janson, D. Cascio and D. Eisenberg, J. Biol. Chem., 264 (1989) 17681.

    CAS  Google Scholar 

  8. J. B. Hunt, P. Z. Smyrniotis, A. Ginsburg and E. R. Stadtman, Arch. Biochem. Biophys., 166 (1975) 102.

    Article  CAS  Google Scholar 

  9. J. B. Hunt and A. Ginsburg, J. Biol. Chem., 255 (1980) 590.

    CAS  Google Scholar 

  10. H. B. Pinkofsky, A. Ginsburg, I. Reardon and R. L. Heinrikson, J. Biol. Chem., 259 (1984) 9616.

    CAS  Google Scholar 

  11. J. J. Villafranca, D. E. Ash and F. C. Wedler, Biochemistry, 15 (1976) 536.

    Article  CAS  Google Scholar 

  12. A. Shrake, M. T. Fisher, P. J. McFarland and A. Ginsburg, Biochemistry, 28 (1989) 6281.

    Article  CAS  Google Scholar 

  13. A. Ginsburg and M. Zolkiewski, Biochemistry, 30 (1991) 9421.

    Article  CAS  Google Scholar 

  14. M. Zolkiewski and A. Ginsburg, Biochemistry, 31 (1992) 11991.

    Article  CAS  Google Scholar 

  15. E. M. Freire and R. L. Biltonen, Biopolymers, 17 (1978) 463.

    Article  CAS  Google Scholar 

  16. E. M. Freire and R. L. Biltonen, Biopolymers, 17 (1978) 481.

    Article  CAS  Google Scholar 

  17. M. Zolkiewski, N. J. Nosworthy and A. Ginsburg, Protein Sci., 4 (1995) 1544.

    CAS  Google Scholar 

  18. N. J. Nosworthy and A. Ginsburg, Protein Sci., 6 (1997) 2617.

    CAS  Google Scholar 

  19. H. Maruta and E. D. Korn, J. Biol. Chem., 252 (1977) 6501.

    CAS  Google Scholar 

  20. T. D. Pollard, W. F. Stafford, III and R. M. E. Porter, J. Biol. Chem., 253 (1978) 4798.

    CAS  Google Scholar 

  21. M. A. L. Atkinson and E. D. Korn, J. Biol. Chem., 261 (1986) 3382.

    CAS  Google Scholar 

  22. J. A. Hammer, III, B. Bowers, B. M. Paterson and E. D. Korn, J. Cell. Biol., 105 (1987) 913.

    Article  CAS  Google Scholar 

  23. T. D. Pollard, J. Cell. Biol., 95 (1982) 816.

    Article  CAS  Google Scholar 

  24. A. D. McLachlan, Annu. Rev. Biophys. Bioeng., 13 (1984) 167.

    Article  CAS  Google Scholar 

  25. C. Ganguly, I. C. Baines, E. D. Korn and J. Sellers, J. Biol. Chem., 267 (1992) 20900.

    CAS  Google Scholar 

  26. M. Zolkiewski, M. J. Redowicz, E. D. Korn and A. Ginsburg, Arch. Biochem. Biophys., 318 (1995) 207.

    Article  CAS  Google Scholar 

  27. M. Zolkiewski, M. J. Redowicz, E. D. Korn and A. Ginsburg, Biophys. Chem., 59 (1996) 365.

    Article  CAS  Google Scholar 

  28. E. Freire, W. W. van Osdol, O. L. Mayorga and J. M. Sanchez-Ruiz, Annu. Rev. Biophys. Chem., 19 (1990) 159.

    Article  CAS  Google Scholar 

  29. J. M. Sanchez-Ruiz, Biophys. J., 61 (1992) 921.

    CAS  Google Scholar 

  30. A. Bertazzon and T. Y. Tsong, Biochemistry, 28 (1989) 9784.

    Article  CAS  Google Scholar 

  31. J. W. Shriver and U. Kamath, Biochemistry, 29 (1990) 2556.

    Article  CAS  Google Scholar 

  32. P. L. Privalov, Adv. Protein Chem., 35 (1982) 1.

    CAS  Google Scholar 

  33. M. Zolkiewski, M. J. Redowicz, E. D. Korn, J. A. Hammer, III and A. Ginsburg, Biochemistry, 36 (1997) 7876.

    Article  CAS  Google Scholar 

  34. I. Rayment, W. R. Rypniewski, K. Schmidt-Base, R. Smith, D. R. Tomchick, M. M. Benning, D. A. Winkelmann, G. Wesenberg and H. M. Holden, Science, 261 (1993) 50.

    CAS  Google Scholar 

  35. I. Rayment, H. M. Holden, M. Whittaker, C. B. Yohn, M. Lorenz, K. C. Holmes and R. A. Milligan, Science, 261 (1993) 58.

    CAS  Google Scholar 

  36. H. M. Warrick and J. A. Spudich, Annu. Rev. Cell. Biol., 3 (1987) 379.

    Article  CAS  Google Scholar 

  37. D. I. Levitsky, V. L. Shnyrov, N. V. Khvorov, A. E. Bukatina, N. S. Vedenkina, E. A. Permyakov, O. P. Nikolaeva and B. F. Poglazov, Eur. J. Biochem., 209 (1992) 829.

    Article  CAS  Google Scholar 

  38. K. S. Thompson, C. R. Vinson and E. Freire, Biochemistry, 32 (1993) 5491.

    Article  CAS  Google Scholar 

  39. N. D. Meadow, D. K. Fox and S. Roseman, Annu. Rev. Biochem., 59 (1990) 497.

    Article  CAS  Google Scholar 

  40. Y. J. Seok, B. R. Lee, P.-P. Zhu and A. Peterkofsky, Proc. Natl. Acad. Sci. USA, 93 (1996) 347.

    Article  CAS  Google Scholar 

  41. N. J. Nosworthy, A. Peterkofsky, S. König, Y.-S. Seok, R. H. Szczepanowski and A. Ginsburg, Biochemistry, 37 (1998) 6718.

    Article  CAS  Google Scholar 

  42. F. Chauvin, A. Fomenkov, C. R. Johnson and S. Roseman, Proc. Natl. Acad. Sci. USA, 93 (1996) 7028.

    Article  CAS  Google Scholar 

  43. A. Ginsburg, R. H. Szczepanowski, S. B. Ruvinov, N. J. Nosworthy, M. Sondeij, T. C. Umland and A. Peterkofsky, Protein Sci., 9 (2000) 1085.

    Article  CAS  Google Scholar 

  44. D.-I. Liao, E. Silverton, Y.-J. Seok, B. R. Lee, A. Peterkofsky and D. R. Davies, Structure, 4 (1996) 861.

    Article  CAS  Google Scholar 

  45. D. S. Garrett, Y.-J. Seok, D.-I. Liao, A. Peterkofsky, A. M. Gronenborn and G. M. Clore, Biochemistry, 36 (1997) 2517.

    Article  CAS  Google Scholar 

  46. D. S. Garrett, Y.-J. Seok, A. Peterkofsky, G. M. Clore and A. M. Gronenborn, Protein Sci., 7 (1998) 789.

    Article  CAS  Google Scholar 

  47. W. Meijberg, G. K. Schuurman-Wolters and G. T. Robillard, Biochemistry, 35 (1996) 2759.

    Article  CAS  Google Scholar 

  48. C. LiCalsi, T. S. Crocenzi, E. Freire and S. Roseman, J. Biol. Chem., 266 (1991) 19519.

    CAS  Google Scholar 

  49. M. E. Huffine and J. M. Scholtz, J. Biol. Chem., 271 (1996) 28898.

    Article  CAS  Google Scholar 

  50. N. A. J. Van Nuland, J. A. Wiersma, D. Van der Spoel, B. L. De Groot, R. M. Scheek and G. T. Robillard, Protein Sci., 5 (1996) 442.

    Article  CAS  Google Scholar 

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  1. Section on Protein Chemistry, Laboratory of Biochemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bldg 3, Room 208, Bethesda, Maryland, 20892-0342, USA

    A. Ginsburg

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Ginsburg, A. Reversible, Thermally Induced Domain Unfolding in Oligomeric Proteins. Spectral and DSC measurements. Journal of Thermal Analysis and Calorimetry 61, 425–436 (2000). https://doi.org/10.1023/A:1010113300190

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