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A serum-free [3H]thymidine incorporation assay for the detection of transforming growth factors

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Journal of tissue culture methods

Summary

A rapid mitogenic assay suitable for the detection of transforming growth factors in the extracts of tissues or cells or in the medium conditioned by tumor cells in vitro is described. The method utilizes a nontumorigenic mouse embryo cell line (AKR-2B cells) maintained in serum-free conditions. Three classes of growth factors can be distinguished using this assay.

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References

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  1. Department of Cell Biology and Anatomy, School of Medicine, The Oregon Health Sciences University, 3181 SW Sam Jackson Park Rd., 97201, Portland, OR

    Gary D. Shipley

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  1. Gary D. Shipley
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Shipley, G.D. A serum-free [3H]thymidine incorporation assay for the detection of transforming growth factors. Journal of Tissue Culture Methods 10, 117–123 (1986). https://doi.org/10.1007/BF01404602

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