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Immortalization of human keratinocytes with human papilloma virus DNA

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Methods in Cell Science

Abstract

Human keratinocytes, derived from the cervix or foreskin, can be immortalized with the HPV-16 or HPV-18 E6 and E7 genes. Two methods of introducing the viral oncogenes into keratinocytes i.e. calcium phosphate transfection and retroviral transduction, are described below, both of which have been optimized for human keratinocytes. While the calcium phosphate transfection method can be used in a normal tissue culture facility, transduction with a retroviral vector containing oncogenes, requires a containment facility and appropriate laboratory practice.

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Authors and Affiliations

  1. Program in Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA

    Christine Halbert

  2. Division of Haematology Virology, Institute of Medical and Veterinary Science, Frome Road, 5000, Adelaide, SA, Australia

    Pritinder Kaur

Authors
  1. Pritinder Kaur
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  2. Christine Halbert
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Kaur, P., Halbert, C. Immortalization of human keratinocytes with human papilloma virus DNA. Methods Cell Sci 17, 117–123 (1995). https://doi.org/10.1007/BF00986660

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  • DOI: https://doi.org/10.1007/BF00986660

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