Summary
This report describes a method for organ culture of the fetal rat lung at the early preglandular phase of development. When the intact fetal lung is cultured fully submerged in medium it develops as an expanded hollow organ. This pattern of in vitro morphogenesis provides a specimen uniquely suited to analysis of the pulmonary epithelium by scanning electron microscopy. In this system alveolar Type II cells and cell types of the distal pulmonary airways undergo differentiation in serum-free media.
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McAteer, J.A., Cavanagh, T.J. & Evan, A.P. Submersion organ culture of preglandular fetal lung. Journal of Tissue Culture Methods 8, 59–62 (1983). https://doi.org/10.1007/BF02099239
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DOI: https://doi.org/10.1007/BF02099239