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Seed specific expression of the 2S albumin gene from Brazil nut (Bertholletia excelsa) in transgenicVicia narbonensis

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Abstract

In order to evaluate the possibility of enhancing the sulphur amino acids in grain legumes, we have transferred the gene for the methionine-rich 2S albumin from Brazil nut (Bertholletia excelsa) controlled by a seed-specific promoter intoVicia narbonensis. The coding region of the 2S albumin gene that has been completely synthesized was fused to the seed-specific leguminB4 promoter fromVicia faba. Transgenic lines ofV. narbonensis were generated viaAgrobacterium-mediated gene analysis of leaves, stems, roots and seeds of four R0 plants revealed that the expression of the foreign 2S albumin gene occurred in a seed-specific manner and that the foreign protein is present at levels ranging from 1% to 4.8% of total SDS-soluble seed protein. Since the current protocol for transformation ofV. narbonensis has not yet been published, a detailed description of the method is given.

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Authors and Affiliations

  1. Institut für Angewandte Genetik, Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195, Berlin, Germany

    Thomas Pickardt, Martin Meixner & Otto Schieder

  2. Institut für Pflanzengenetik und Kulturpflanzenforschung, 06466, Gatersleben, Germany

    Isolde Saalbach, David Waddell & Klaus Müntz

Authors
  1. Thomas Pickardt
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  2. Isolde Saalbach
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  3. David Waddell
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  4. Martin Meixner
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  5. Klaus Müntz
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  6. Otto Schieder
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Pickardt, T., Saalbach, I., Waddell, D. et al. Seed specific expression of the 2S albumin gene from Brazil nut (Bertholletia excelsa) in transgenicVicia narbonensis . Mol Breeding 1, 295–301 (1995). https://doi.org/10.1007/BF02277429

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  • DOI: https://doi.org/10.1007/BF02277429

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