Abstract
Latexin, a carboxypeptidase A inhibitor, is expressed in a cell type-specific manner in both central and peripheral nervous systems in the rat. It is used as a molecular marker for the regional specification of the neocortex. In this study, a cDNA was isolated from a human fetal brain cDNA library. The cDNA (LXN) contains an open reading frame encoding 222 amino acids. The comparison between the deduced amino acid sequences of LXN and latexins of rat and mouse revealed high sequence identity (84.2 and 84.7%, respectively). Northern blot analysis showed that LXN was expressed as a transcript of 1.3 kb in 15 out of 16 tissues examined, except in peripheral blood leukocyte. The expression levels were high in heart, prostate, ovary, kidney, pancreas, and colon, moderate or low in other tissues including brain. It is noteworthy that the tissue distribution of human LXN differs greatly to that of its homologue in the model animal, rat latexin. In addition, the LXN gene contains at least 6 exons and spans 5.9 kb according to the genomic sequence of the clone RP11-79M21 and the gap sequence cloned in this paper. LXN was assigned to 3q25-q26.2 according to the position of the marker SHGC-35682 found adjacent to LXN gene.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Rakic P (1988) Science 241: 170–176.
Levitt P, Barbe MF & Eagleson KL (1997) Annu. Rev. Neurosci. 20: 1–24.
Arimatsu Y, Nihonmatsu I, Hirata K & Takiguchi-Hayashi K (1994) J. Neurosci. 14: 2020–2031.
Arimatsu Y, Miyamoto M, Nihonmatsu I, Hirata K, Uratani Y, Hatanaka Y & Takiguchi-Hayashi K (1992) Proc. Natl. Acad. Sci. USA 89: 8879–8883.
Arimatsu Y (1994) Neurosci. Res. 20: 131–135.
Arimatsu Y & Ishida M (1988) Brain Res. Dev. Brain Res. 106: 71–78.
Arimatsu Y, Ishida M, Takiguchi-Hayashi K & Uratani Y (1999) Development 126: 629–638.
Arimatsu Y, Kojima M & Ishida M (1999) Neuroscience 88: 93–105.
Normant E, Martres M.-P, Schwartz J-C & Gros C (1995) Proc. Natl. Acad. Sci. USA 92: 12225–12229.
Hatanaka Y, Uratani Y, Takiguchi-Hayashi K, Omori A, Sato K, Miyamoto M & Arimatsu Y. (1994) Eur. J. Neurosci. 6: 973–982.
Jin M-h, Uratani Y & Arimatus Y (1997) Genomics 39: 419–421.
DeBry RW & Seldin MF (1996) Genomics 33: 337–351.
Miyasaka N, Hananaka Y, Jin M & Arimatsu Y (1999) Brain Res. Mol. Brain. Res. 69: 62–72.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liu, Q., Yu, L., Gao, J. et al. Cloning, tissue expression pattern and genomic organization of latexin, a human homologue of rat carboxypeptidase A inhibitor. Mol Biol Rep 27, 241–246 (2000). https://doi.org/10.1023/A:1010971219806
Issue Date:
DOI: https://doi.org/10.1023/A:1010971219806