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Narbonin, a novel 2S protein from Vicia narbonensis L. seeds: cDNA, gene structure and developmentally regulated formation

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Abstract

cDNA and genomic clones encoding narbonin, a 2S globulin from the seed of narbon bean (Vicia narbonensis L.), were obtained using the polymerase chain reaction (PCR) and sequenced. The full-length cDNA as well as genomic clones contain a single open reading frame (ORF) of 873 bp that encodes a protein with 291 amino acids comprising the mature narbonin polypeptide (M r ca. 33 100) and an initiation methionine. The deduced amino acid sequence lacks a transient N-terminal signal peptide. The genomic clones do not contain any intron. No homology was found to nucleic acid and protein sequences so far registered in sequence data libraries. The biosynthesis of narbonin during embryogenesis is developmentally-regulated and its pattern of synthesis closely resembles that of typical seed storage globulins. However, during seed germination narbonin was degraded very slowly, indicating that it may have other function than storage protein. Southern analysis suggests the existence of a small narbonin gene family. Narbonin genes were also found in four different species of the genus Vicia as well as in other legumes such as Canavalia ensiformis and Glycine max. In Escherichia coli a recombinant narbonin was produced which yielded crystals like those prepared from narbonin purified from seeds.

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Author notes

  1. Ronald Bassüner

    Present address: Dept. of Agronomy, Purdue University, 47906, West Lafayette, IN, USA

Authors and Affiliations

  1. Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466, Gatersleben, Germany

    Hai Van Nong, Bernhard Schlesier, Ronald Bassüner, Alexander Repik, Christian Horstmann & Klaus Müntz

  2. Institute of Biotechnology, National Center for Natural Science and Technology, Hanoi, Vietnam

    Hai Van Nong

Authors
  1. Hai Van Nong
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  2. Bernhard Schlesier
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  3. Ronald Bassüner
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  4. Alexander Repik
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  5. Christian Horstmann
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  6. Klaus Müntz
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Van Nong, H., Schlesier, B., Bassüner, R. et al. Narbonin, a novel 2S protein from Vicia narbonensis L. seeds: cDNA, gene structure and developmentally regulated formation. Plant Mol Biol 28, 61–72 (1995). https://doi.org/10.1007/BF00042038

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  • DOI: https://doi.org/10.1007/BF00042038

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