Abstract
Phytolacca anti-viral protein (PAP) was purified from Phytolacca leaves and the N-terminal was sequenced. A cDNA library was made from mRNAs isolated from Phytolacca leaves and cDNA clones for PAP were identified using oligonucleotide probes derived from the N-terminal amino acid sequence. The PAP-cDNA clone was sequenced from both directions. The predicted amino acid sequence of PAP was compared with the amino acid sequences of other ribosome-inactivating proteins. The identities of these proteins to PAP ranged from 29 to 38%, and a region was found in each with a sequence similar to the PAP sequence (AIQMVSEAARFKYI). Southern blot analysis indicates that PAP is encoded by a multi-gene family.
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Abbreviations
- MAP:
-
Mirabilis jalapa anti-viral protein
- PAP:
-
Phytolacca anti-viral protein
- SO6:
-
30 kDa ribosome-inactivating protein from the seeds of Saponaria officinalis
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Lin, Q., Chen, Z.C., Antoniw, J.F. et al. Isolation and characterization of a cDNA clone encoding the anti-viral protein from Phytolacca americana . Plant Mol Biol 17, 609–614 (1991). https://doi.org/10.1007/BF00037047
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DOI: https://doi.org/10.1007/BF00037047