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A complete cDNA for adenine phosphoribosyltransferase fromArabidopsis thaliana

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Abstract

An intact cDNA fromArabidopsis thaliana for adenine phosphoribosyltransferase (APRT) was isolated and sequenced. The cDNA is 729 nucleotides in length and predicts a protein ofM r 27140. The deduced amino acid sequence has been compared with those of other APRTs and shown to be most similar to theEscherichia coli protein. Construction of a molecular tree of the known APRT amino acid sequences indicates theA. thaliana andE. coli APRT sequences form one cluster and the currently available vertebrate and invertebrate sequences form a separate grouping. Since it is possible to select either for or against the expression of APRT, the isolation of this APRT cDNA clone will allow these selection schemes to be used in plant genetic experiments.

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Authors and Affiliations

  1. Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ont., Canada

    B. A. Moffatt, E. A. McWhinnie & J. J. Pasternak

  2. Glaxo Research Laboratories, Venture Center Building, 4117 Emperor Blvd., 27560, Morrisville, NC, USA

    W. E. Burkhart

  3. Department of Molecular Biology and Genetics, University of Guelph, N1G 2W1, Guelph, Ont., Canada

    S. J. Rothstein

Authors
  1. B. A. Moffatt
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  2. E. A. McWhinnie
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  3. W. E. Burkhart
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  4. J. J. Pasternak
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  5. S. J. Rothstein
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Moffatt, B.A., McWhinnie, E.A., Burkhart, W.E. et al. A complete cDNA for adenine phosphoribosyltransferase fromArabidopsis thaliana . Plant Mol Biol 18, 653–662 (1992). https://doi.org/10.1007/BF00020008

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  • DOI: https://doi.org/10.1007/BF00020008

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