Abstract
The changes in the levels of α-amylase mRNA is barley aleurone layers in response to addition of plant growth regulators have been studied using a cloned α-amylase cDNA as the hybridization probe. An increase in gibberellic acid (GA) concentration in the incubation medium from 10−9 M to 10−6 M results in a progressive increase in α-amylase mRNA concentration in the aleurone cells. Detectable levels of α-amylase mRNA appear in the aleurone cells as early as 1 h after addition of GA. The concentration of this mRNA increases for several hours and then declines rapidly. Abscisic acid (ABA) and the amino acid analog S-2-aminoethyl-L-cysteine (AEC) suppress the GA-mediated induction of α-amylase. These compounds appear to affect the level of α-amylase mRNA in aleurone cells as measured byin vitro translation assays and by analysis of RNA blots with α-amylase cDNA probes. It is concluded that the regulation of α-amylase gene expression by ABA is at the level of transcription. Further, a protein factor appears to be required in addition to GA for transcription of α-amylase genes.
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Bennet PA, Chrispeels MJ: De novo synthesis of ribonuclease and β-1,3-glucanase by aleurone cells of barley. Plant Physiol 49:455–457, 1972.
Bernal-Lugo I, Beachy RN, Varner JE: The response of barley aleurone layers to gibberellic acid includes the transcription of new sequences. Biochem Biophys Res Commun 102:617–623, 1972.
Callis J, Ho DTH: Multiple molecular forms of the gibberllin-induces α-amylase from the aleurone layers of barley seeds. Arch Biochem Biophys 224:224–234, 1983.
Chrispeels MJ, Varner JE: Inhibition of gibberellic acid-induced formation of α-amylase by abscisin II. Nature 212:1066–1067, 1966.
Filner P, Varner JE: A test for de novo synthesis of enzymes: density labeling with H2O18 of barley α-amylase induced by gibberellic acid. Proc Natl Acad Sci USA 58:1520–1526, 1967.
Higgins TJV, Jacobsen JV, Zwar JA: Gibberellic acid and abscisic acid modulate protein synthesis and mRNA levels in barley aleurone layers. Plant Molec Biol 1:191–215, 1982.
Higgins TJV, Zwar JA, Jacobsen JV: Gibberellic acid enhances the level of translatable mRNA for α-amylase in barley aleurone layers. Nature 260:166–168, 1976.
Ho DTH: Hormonal and genetic regulation of α-amylase synthesis in barley aleurone cells. In: Leaver CJ (ed) Genome Organization and Expression in Plants. Plenum Press, New York, 1980, pp 147–156.
Ho DTH, Varner JE: Hormonal control of messenger ribonucleic acid metabolism in barley aleurone layers. Proc Natl Acad Sci USA 71:4783–4786, 1974.
Ho DTH, Varner JE: Response of barley aleurone layers to abscisic acid. Plant Physiol 57:175–178, 1976.
Jacobsen JV, Higgins TJV: Characterization of the α-amylases synthesized by aleurone layers of Himalaya barley in response to gibberellic acid. Plant Physiol 70:1647–1653, 1982.
Jacobsen JV, Higgins TJV, Zwar JA: Hormonal control of endosperm function during germination. In Rubinstein I (ed) The Plant Seed—Development, Preservation, and Germination. Academic Press, New York, 1979, pp 241–262.
Jacobsen JV, Zwar JA: Gibberellic acid causes increased synthesis of RNA which contains poly(A) in barley aleurone tissue. Proc Natl Acad Sci USA 71:3290–3293, 1974.
Jones RL, Jacobsen JV: Membrane and RNA metabolism in the response of aleurone cells to GA. Bot Mag Tokyo Special Issue 1:83–99, 1978.
Jones RL, Varner JE: The bioassay of gibberellins. Planta (Berl) 72:155–161, 1967.
Mathewson PR, Pomeranz Y: Modified chromogenic α-amylase assay for sprouted wheat. J Assoc Off Anal Chem 62:198–200, 1979.
Miyata S, Okamoto K, Watanabe A, Akazawa T: Enzymatic mechanism of starch breakdown in germinating rice seeds. In vivo and in vitro synthesis of α-amylase in rice scutellum. Plant Physiol 68:1314–1318, 1981.
Muthukrishnan S, Chandra GR: Expression of α-amylase genes in barley aleurone cells. Beltsville Symposia on Agricultural Research VII, 1983, in press.
Muthukrishnan S, Chandra GR, Maxwell ES: Hormone-induced increase in levels of functional mRNA and α-amylase mRNA in barley aleurones. Proc Natl Acad Sci USA 76:6181–6185, 1979.
Muthukrishnan S, Chandra GR, Maxwell ES: Hormonal control of α-amylase gene expression in barley. Studies using a cloned cDNA probe. J Biol Chem 258:2370–2375, 1983.
Mozer T: Control of protein synthesis in barley aleurone layers by the plant hormones gibberellic acid and abscisic acid. Cell 20:479–485, 1980.
Okita TW, DeCaleya R, Rappaport L: Synthesis of a possible precursor of α-amylase in wheat aleurone cells. Plant Physiol 63: 195–200, 1979.
Rogers JC, Milliman C: Isolation and sequence analysis of a barley α-amylase cDNA clone. J Biol Chem 258:8169–8174, 1983.
Thomas P: Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77:5201–5205, 1980.
Yomo H, Varner JE: Hormonal control of a secretory tissue. Current Topics in Develop Biol 6:111–144, 1971.
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Muthukrishnan, S., Chandra, G.R. & Albaugh, G.P. Modulation by abscisic acid and S-2-aminoethyl-L-cysteine of α-amylase mRNA in barley aleurone cells. Plant Mol Biol 2, 249–258 (1983). https://doi.org/10.1007/BF01578643
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DOI: https://doi.org/10.1007/BF01578643