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Deviating T-DNA transfer fromAgrobacterium tumefaciens to plants

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Abstract

We analyzed 29 T-DNA inserts in transgenicArabidopsis thaliana plants for the junction of the right border sequences and the flanking plant DNA. DNA sequencing showed that in most lines the right border sequences transferred had been preserved during integration, corroborating literature data. Surprisingly, in four independent transgenic lines a complete right border repeat was present followed by binary vector sequences. Cloning of two of these T-DNA inserts by plasmid rescue showed that in these lines the transferred DNA consisted of the complete binary vector sequences in addition to the T-region. On the basis of the structure of the transferred DNA we propose that in these lines T-DNA transfer started at the left-border repeat, continued through the vector part, passed the right border repeat, and ended only after reaching again this left-border repeat.

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Authors and Affiliations

  1. Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, Wasenaarseweg 64, 2333 AL, Leiden, Netherlands

    Eric van der Graaff, Amke den Dulk-Ras & Paul J. J. Hooykaas

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  1. Eric van der Graaff
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  2. Amke den Dulk-Ras
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  3. Paul J. J. Hooykaas
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van der Graaff, E., den Dulk-Ras, A. & Hooykaas, P.J.J. Deviating T-DNA transfer fromAgrobacterium tumefaciens to plants. Plant Mol Biol 31, 677–681 (1996). https://doi.org/10.1007/BF00042239

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  • DOI: https://doi.org/10.1007/BF00042239

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