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cDNA and protein sequence of a major pea seed albumin (PA 2 : Mr≈26 000)

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Summary

Pea albumin 2 (PA2:Mr≈26000) is a major component of the albumin fraction derived from aqueous salt extracts of pea seed. Sodium dodecylsulfate-polyacrylamide gel electrophoresis and chromatography on DEAE-Sephacel resolve PA2 into two closely related components (PA2a and PA2b). A cDNA clone coding for one of these components has been sequenced and the deduced amino acid sequence compared with partial, chemically-determined sequences for cyanogen bromide peptides from both PA2 components. Complete amino acid sequences were obtained for the C-terminal peptides. The PA2 molecule of 230 amino acids contains four imperfect repeat sequences each of approximately 57 amino acids in length.

The combined sequence data, together with a comparison of PA2-related polypeptides produced in vitro and in vivo, indicate that PA2 is synthesized without a signal sequence and does not undergo significant post-translational modification. Although both forms of PA2 contain Asn-X-Thr consensus sequences, neither form is glycosylated. Accumulation of PA2 contributes approximately 11% of the sulfur-amino acids in pea seeds (cysteine plus methionine equals 2.6 residues percent). Suppression of levels of PA2 polypeptides and their mRNAs in developing seeds of sulfur-deficient plants is less marked than that for legumin, in spite of the lower content of sulfur-amino acids in legumin.

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References

  1. Argos P, Pedersen K, Marks MD, Larkins BA: A structural model for maize zein proteins. J Biol Chem 257: 9984–9990, 1982.

    Google Scholar 

  2. Badenoch-Jones J, Spencer D, Higgins TJV, Millerd A: The role of glycosylation in storage protein synthesis in developing pea seeds. Planta 153: 210–209, 1981.

    Google Scholar 

  3. Bartels D, Thompson RD: The characterization of cDNA clones for wheat storage proteins. Nucleic Acids Res 11: 2961–2977, 1983.

    Google Scholar 

  4. Blagrove RJ, Gillespie JM, Randall PJ: Effect of sulfur supply on the seed globulin composition of Lupinus angustifolius. Aust J Plant Physiol 3: 173–184, 1976.

    Google Scholar 

  5. Chandler PM: The use of single-stranded phage DNAs in hybrid arrest and release translation. Anal Biochem 127: 9–16, 1982.

    Google Scholar 

  6. Chandler PM, Higgins TJV, Randall PJ, Spencer D: Regulation of legumin levels in developing pea seeds under conditions of sulfur deficiency. Plant Physiol 71: 47–54, 1983.

    Google Scholar 

  7. Chandler PM, Spencer D, Randall PJ, Higgins TJV: Influence of sulfur nutrition on developmental patterns of some major pea seed proteins and their mRNAs. Plant Physiol 75: 651–657, 1984.

    Google Scholar 

  8. Chrispeels MJ, Baumgarter B, Harris N: Regulation of reserve protein metabolism in cotyledons of mung bean seedlings. Proc Natl Acad Sci USA 73: 3168–3172, 1976.

    Google Scholar 

  9. Croy RRD, Hoque MS, Gatehouse JA, Boulter D: The major albumin proteins from pea (Pisum sativum L). Biochem J 218: 795–803, 1984.

    Google Scholar 

  10. Gatehouse JA; Gilroy J, Hoque MS, Croy RRD: Purification, properties and amino acid sequence of a low-Mr abundant seed protein from pea (Pisum sativum L). Biochem J 225: 239–247, 1985.

    Google Scholar 

  11. Geraghty D, Peifer MA, Rubenstein I, Messing J: The primary structure of a plant storage protein: zein. Nucleic Acids Res 9: 5163–5174, 1981.

    Google Scholar 

  12. Gross E, Witkop B: Non-enzymatic cleavage of peptide bonds: the methionine residues in bovine pancreatic ribonuclease. J Biol Chem 237: 1856–1860, 1962.

    Google Scholar 

  13. Harris N, Croy RRD: The major albumin from pea (Pisum sativumL). Localization and immunocytochemistry. Planta 165: 522–526, 1985.

    Google Scholar 

  14. Higgins TJV: Synthesis and regulation of major proteins in seeds. Annu Rev Plant Physiol 35: 191–221, 1984.

    Google Scholar 

  15. Higgins TJV, Chandler PM, Randall PJ, Spencer D, Beach LR, Blagrove RJ, Kortt AA, Inglis AS: Gene structure, protein sequence and regulation of the synthesis of a sulfur-rich protein in pea seeds. J Biol Chem 1986. In press.

  16. Inglis AS, Strike PM, Osborne WC, Burley RW: Sequenator determination of the amino acid sequence of apovitellinin 1 from turkey's egg yolk. Use of a stationary reaction cup during peptide bond cleavage. FEBS Lett 97: 179–182, 1979.

    Google Scholar 

  17. Kortt AA, Burns JE, Trinnick MJ, Appleby CA: The amino acid sequence of hemoglobin from Parasponia andersonii, a non-leguminous plant. FEBS Lett 180: 144–150, 1985.

    Google Scholar 

  18. Millerd A, Spencer D: Changes in RNA-synthesizing activity and template activity in nuclei from cotyledon of developing pea seeds. Aust J Plant Physiol 1: 331–341, 1974.

    Google Scholar 

  19. Murray DR: A storage role for albumins in pea cotyledons. Plant, Cell and Environment 2: 221–226, 1979.

    Google Scholar 

  20. Perlman D, Halvorsen HO: A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides. J Mol Biol 167: 391–409, 1983.

    Google Scholar 

  21. Peterson JD, Nehrlich S, Dyer PE, Steiner DF: Determination of the amino acid sequence of the monkey, sheep and dog proinsulin C-peptides by a semi-micro Edman degradation procedure. J Biol Chem 247: 4866–4871, 1972.

    Google Scholar 

  22. Randall PJ, Thomson JA, Schroeder HE: Cotyledonary storage proteins in Pisum sativum. IV Effects of sulfur, phosphorus, potassium and magnesium deficiencies. Aust J Plant Physiol 6: 11–24, 1979.

    Google Scholar 

  23. Sanger F, Nicklen S, Coulen AR: DNA sequencing with chain terminating inhibitors Proc Natl Acad Sci USA 74: 5463–5467, 1977.

    Google Scholar 

  24. Schroeder HE: Quantitative studies on the cotyledonary proteins of the genus Pisum. J Sci Food Agric 33: 623–633, 1982.

    Google Scholar 

  25. Schroeder HE: Major albumins of Pisum cotyledons. J Sci Food Agric 35: 191–198, 1984.

    Google Scholar 

  26. Shewry PJ, Franklin J, Parmer S, Smith SJ, Miflin BJ: The effects of sulfur starvation on the amino acid and protein compositions of barley grain. J Cereal Sci 1: 21–31, 1983.

    Google Scholar 

  27. Spencer D, Higgins TJV, Button SC, Davey RA: Pulselabelling studies on protein synthesis in developing pea seeds and evidence of a precursor form of legumin small subunit. Plant Physiol 66: 510–515, 1980.

    Google Scholar 

  28. Struck DK, Lennarz WJ, Brew K: Primary structural requirements for the enzymatic formation of the N-glycosidic bond in glycoproteins. J Biol Chem 253: 5786–5794, 1978.

    Google Scholar 

  29. Woods EF, Inglis AS: Organization of the coiled-coils in the wool microfibril. Int J Biol Macromol 6: 277–283, 1984.

    Google Scholar 

  30. Wrigley CW, duCros DL, Archer MJ, Downie PJ, Roxburgh CM: The sulfur content of wheat endosperm proteins and its relevance to grain quality. Aust J Plant Physiol 7: 755–766, 1980.

    Google Scholar 

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Authors and Affiliations

  1. Division of Plant Industry, CSIRO, GPO Box 1600, 2601, Canberra, A.C.T., Australia

    Thomas J. V. Higgins, Larry R. Beach, Donald Spencer, Peter M. Chandler & Peter J. Randall

  2. Division of Protein Chemistry, CSIRO, 343 Royal Parade, 3052, Parkville, Victoria, Australia

    Robert J. Blagrove, Alexander A. Kortt & Robin E. Guthrie

Authors
  1. Thomas J. V. Higgins
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  2. Larry R. Beach
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  3. Donald Spencer
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  4. Peter M. Chandler
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  5. Peter J. Randall
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  6. Robert J. Blagrove
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  7. Alexander A. Kortt
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  8. Robin E. Guthrie
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Higgins, T.J.V., Beach, L.R., Spencer, D. et al. cDNA and protein sequence of a major pea seed albumin (PA 2 : Mr≈26 000). Plant Mol Biol 8, 37–45 (1987). https://doi.org/10.1007/BF00016432

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  • DOI: https://doi.org/10.1007/BF00016432

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