Skip to main content
SpringerLink
Log in

Effect of abscisic and gibberellic acids on malate synthase transcripts in germinating castor bean seeds

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

Several clones complementary to malate synthase mRNA have been identified in a complementary-DNA library to mRNA from castor bean endosperm. One of these clones has been used as a probe to measure levels of transcripts during seed germination and the effects of gibberellic acid and abscisic acid on these levels have been examined.

Malate synthase transcripts increased during germination and GA3 advanced their appearance in the endosperm. Exogenously applied ABA inhibited the accumulation of transcripts over a time course of germination but the addition of GA3 counteracted its inhibitory effects. The data confirmed previous reports which indicated that the action of both growth regulators was on transcript accumulation and that there is a coordinated induction of the enzymes involved in the lipid metabolism in oil seeds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ABA:

abscisic acid

GA3 :

gibberellic acid

poly(A+)RNA:

polyadenylated RNA

SDS-PAGE:

sodium dodecyl sulphate-polyacrylamide gel electrophoresis

References

  1. Beevers H: Glyoxysomes of castor bean endosperm and their relation with gluconeogenesis. Ann NY Acad Sci 168: 313–324 (1969).

    Google Scholar 

  2. Beeching JR, Northcote DH: Nucleic acid (cDNA) and amino acid sequences of isocitrate lyase from castor bean. Plant Mol Biol 8: 471–475 (1987).

    Google Scholar 

  3. Bernal-Lugo I, Beachy RN, Varner JE: The response of barley aleurone layers to gibberellic acid includes the transcription of new sequences. Biochem Biophys Res Comm 102: 617–623 (1981).

    Google Scholar 

  4. Bowden L, Lord JM: Purification and comparative properties of microsomal and glyoxysomal malate synthase from castor bean endosperm. Plant Physiol 61: 259–265 (1978).

    Google Scholar 

  5. Choinski JSJr., Trelease RN, Doman DC: Control of enzyme activities in cotton cotyledons during maturation and germination. III. In vitro embryo development in the presence of abscisic acid. Planta 152: 428–435 (1981).

    Google Scholar 

  6. Clements MJ: Purification of eukaryotic messenger RNA. In: Transcription and translation. A practical approach. IRL Press, Oxford, England (1984) pp 381–400.

    Google Scholar 

  7. Crouch ML, Tenbarge K, Simon A, Finkelstein R, Scofield S, Solberg L: Storage protein mRNA levels can be regulated by abscisic acid in Brassica embryos. In: vanVloten-Doting L, Groot GSP, Hall TC (eds). Molecular form and function of the plant genome. Plenum, NY (1985) pp 555–566.

    Google Scholar 

  8. Dommes J, Northcote DH: The action of exogenous abscisic acid and gibberellic acid on gene expression in germinating castor bean. Planta 165: 513–521 (1985).

    Google Scholar 

  9. Dommes J, Northcote DH: The action of exogenous abscisic acid on malate-synthase synthesis in germinating castor-bean seeds. Planta 166: 550–556 (1985).

    Google Scholar 

  10. Dretzen G, Ballard M, Sassone-Corsi P, Chambon P: A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem 112: 295–298 (1981).

    Google Scholar 

  11. Finkelstein RR, Tenbarge KM, Shumway JE, Crouch ML: Role of ABA in maturation of rape seed embryos. Plant Physiol 78: 630–636 (1985).

    Google Scholar 

  12. Grunstein M, Hogness D: Colony hybridisation. A method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72: 3961–3965 (1975).

    Google Scholar 

  13. Hardy KJ, Peterlin BM, Atchison RE, Stobo JD: Regulation of expression of the human interferon gamma gene. Proc Natl Acad Sci USA 82: 8173–8177 (1985).

    Google Scholar 

  14. Ho T-HD: On the mode of action of abscisic acid in barley aleurone cells (Abstr.) Plant Physiol 63 Suppl 79 (1979).

    Google Scholar 

  15. Ihle JN, Dure LIII: Hormonal regulation of translation inhibition requiring RNA synthesis. Biochem Biophys Res Comm 38: 995–1001 (1970).

    Google Scholar 

  16. Ihle JN, Dure LIII: Developmental biochemistry of cotton-seed embryogenesis and germination. III. Regulation of the biosynthesis of enzymes utilized in germination. J Biol Chem 247: 5048–5055 (1972).

    Google Scholar 

  17. Jacobsen JV: Regulation of protein synthesis in aleurone cells by gibberellic and abscisic acid. In: Crozier A (ed.) The Biochemistry and Physiology of Gibberellins. New York, Praeger (1984) Vol. 2, pp 159–187.

    Google Scholar 

  18. Jacobsen JV, Beach LR: Control of transcription of α-amylase and rRNA genes in barley aleurone protoplasts in gibberellic and abscisic acid. Nature (London) 316: 275–277 (1985).

    Google Scholar 

  19. Kindl H: Glyoxysome biogenesis via cytosolic pools in cucumber. Ann NY Acad Sci 386: 314–328 (1982).

    Google Scholar 

  20. Maniatis T, Fritsch EF, Sambrook J: Molecular cloning —a laboratory manual. Cold Spring Harbor Laboratory, NY (1982).

    Google Scholar 

  21. Marriott KM, Northcote DH: The induction of enzyme activity in the endosperm of germinating castor-bean seeds. Biochem J 152: 65–70 (1975).

    Google Scholar 

  22. Marriott KM, Northcote DH: The influence of abscisic acid, adenosine 3′, 5′ cyclic phosphate and gibberellic acid on the induction of isocitrate lyase activity in the endosperm of germinating castor bean seeds. J Exp Bot 28: 219–224 (1977).

    Google Scholar 

  23. Martin C, Beeching JR, Northcote DH: Changes in levels of transcripts in endosperms of castor bean treated with exogenous gibberellic acid. Planta 162: 68–76 (1984).

    Google Scholar 

  24. Martin C, Northcote DH: Qualitative and quantitative changes in mRNA of castor bean during the initial stages of germination. Planta 151: 189–197 (1981).

    Google Scholar 

  25. Martin C, Northcote DH: The action of exogenous gibberellic acid on isocitrate lyase-mRNA in germinating castor bean seeds. Planta 154: 174–183 (1982).

    Google Scholar 

  26. Milborrow BV: The chemistry and physiology of abscisic acid. Ann Rev Plant Physiol 25: 259–307 (1974).

    Google Scholar 

  27. Mozer TJ: Control of protein synthesis in barley aleurone layers by the plant hormones gibberellic acid and abscisic acid. Cell 20: 479–485 (1980).

    Google Scholar 

  28. Mundy J: Hormonal regulation of α-amylase inhibitor synthesis in germinating barley. Carlsberg Res Commun 49: 439–444 (1984).

    Google Scholar 

  29. Muthukrishman S, Chandra GR, Albaugh GP: Modulation by abscisic acid and S-2-amynoethyl-L-cysteine of α-amylase mRNA in barley aleurone cells. Plant Mol Biol 2: 249–258 (1983).

    Google Scholar 

  30. Quatrano RS, Ballo BL, Williamson JD, Hamblin MT, Mansfield M: ABA controlled expression of embryospecific genes during wheat grain development. In: Goldberg R (ed.) Plant Molecular Biology. Liss Inc New York (1983) pp 343–353.

    Google Scholar 

  31. Rodriguez D, Nicolas G, Aldasoro JJ, Hernandez-Nistal J, Babiano MJ, Matilla A: Altered development of polysomal RNA activity in chick-pea (Cicer arietinum L.) embryonic axes. Effects of abscisic acid and temperature. Planta 164: 517–523 (1985).

    Google Scholar 

  32. Sham M-H, Northcote DH: Transcription and translation of phloem protein (PP2) during phloem differentiation in Cucurbita maxima. Planta 170: 392–399 (1987).

    Google Scholar 

  33. Smith SM, Leaver CJ: Glyoxysomal malate synthase of cucumber: molecular cloning of a cDNA and regulation of enzyme synthesis during germination. Plant Physiol 81: 762–767 (1986).

    Google Scholar 

  34. Thomas PS: Hybridisation of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77: 5201–5205 (1980).

    Google Scholar 

  35. Varty K, Arreguin LB, Gomez TM, Lopez TPJ, Gomez LMA: Effects of abscisic acid and ethylene on the gibberellic acid-induced synthesis of α-amylase by isolated wheat aleurone layers. Plant Physiol 73: 692–697 (1982).

    Google Scholar 

  36. Weir EM, Riezman H, Grienenberger JM, Becker WM, Leaver CJ: Regulation of glyoxysomal enzymes during germination of cucumber. Temperal changes in translatable mRNA for isocitrate lyase and malate synthase. Eur J Biochem 112: 469–477 (1980).

    Google Scholar 

Download references

Author information

Author notes

  1. J. Dommes

    Present address: Laboratoire de Physiologie, Départment de Botanique, B22, Université de Liège, Sart Tilman, B-4000, Liège, Belgium

Authors and Affiliations

  1. Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW, Cambridge, UK

    D. Rodriguez, J. Dommes & D. H. Northcote

Authors
  1. D. Rodriguez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Dommes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. H. Northcote
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rodriguez, D., Dommes, J. & Northcote, D.H. Effect of abscisic and gibberellic acids on malate synthase transcripts in germinating castor bean seeds. Plant Mol Biol 9, 227–235 (1987). https://doi.org/10.1007/BF00166459

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00166459

Key words

Search

Navigation