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Characterization of a cDNA encoding ricin E, a hybrid ricin-Ricinus communis agglutinin gene from the castor plant Ricinus communis

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Abstract

Two classes of ricin cDNA clones have been identified and sequenced. The cDNA clone pBL-1 closely matches in nucleotide sequence the ricin genomic clone pAKG previously described by Halling et al., 1985 (Nucl. Acids Res. 13:8019). A second group of cDNA clones, represented by pBL-3, encode a hybrid protein (ricin E), having a ricin-like A chain and N-terminal half of the B chain and an RCA (Ricinus communis agglutinin)-like C-terminal half of the B chain.

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References

  1. Alwine JC, Kemp DJ, Parker BA, Reiser J, Renart J, Stark GR, Wahl: Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. In: Wu R (ed.) Methods in Enzymology, Vol. 68, pp. 220–242. New York: Academic Press (1979).

    Google Scholar 

  2. Araki T, Funatsu G: The complete amino acid sequence of the B-chain of ricin E isolated from small-grain castor bean seeds. Ricin E is a gene recombination product of ricin D and Ricinus communis agglutinin. Biochim Biophys Acta 911: 191–200 (1987).

    Google Scholar 

  3. Araki T, Yoshioka Y, Funatsu G: The complete amino acid sequence of the B chain of the Ricinus communis agglutinin isolated from large grain castor bean seeds. Biochim Biophys Acta 872: 277–285 (1986).

    Google Scholar 

  4. Aviv H, Leder P: Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid cellulose. Proc Natl Acad Sci 69: 1408–1412 (1972).

    Google Scholar 

  5. Bailey JM, Davidson N. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry 70: 75–85 (1976).

    Google Scholar 

  6. Cawley DB, Hedblom ML, Houston LL: Homology between ricin and Ricinus communis agglutinin: Amino terminal sequence analysis and protein synthesis inhibition studies. Arch Biochem Biophys 190: 744–755.

  7. Funatsu G, Kimura M, Funatsu M: Primary structure of Ala chain of Ricin D. Agric Biol Chem 43: 2221–2224 (1979).

    Google Scholar 

  8. Funatsu G, Funatsu M: Separation of the two constituent polypeptide chains of ricin D. Agric Biol Chem 41: 1211–1215 (1977).

    Google Scholar 

  9. Funatsu G, Yoshitake S, Funatsu M: Primary structure of Ile chain of Ricin D. Agric Biol Chem 42: 501–503 (1978).

    Google Scholar 

  10. Grunstein M, Hogness DS: Colony hybridization: A method for the isolation of cloned cDNAs that contain a specific gene. Proc Natl Acad Sci 72: 3961–3965 (1975).

    Google Scholar 

  11. Halling KC, Halling AC, Murray EE, Ladin BF, Houston LL, Weaver RF: Genomic cloning and characterization of a ricin gene from Ricinus communis. Nucleic Acids Res 13: 8019–8033 (1985).

    Google Scholar 

  12. Hedblom ML, Cawley DB, Houston LL: The specific binding of ricin and its polypeptide chains to rat liver ribosome and ribosomal subunit. Arch Biochem Biophys 177: 46–55 (1976).

    Google Scholar 

  13. Lamb FI, Roberts LM, Lord JM: Nucleotide sequence of cloned cDNA coding for preproricin. Eur J Biochem 148: 265–270 (1985).

    Google Scholar 

  14. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning—A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.

  15. Maxam AM, Gilbert W: A new method for sequencing DNA. Proc Natl Acad Sci 74: 560–564 (1977).

    Google Scholar 

  16. Messing J, Vieira J: A new pair of M13 vectors for selecting either strand of a double-digest restriction fragment. Gene 19: 269–276 (1982).

    Google Scholar 

  17. Mise T, Funatsu G, Ishiguro M, Funatsu M: Isolation and characterization of ricin E from castor beans. Agric Biol Chem 41: 2041–2046 (1977).

    Google Scholar 

  18. Nakamichi N, Rhoads DD, Roufa DJ: The Chinese hamster cell emetine resistance gene. Analysis of cDNA and genomic sequences encoding ribosomal protein S14. J Biol Chem 258: 13236–13242 (1983).

    Google Scholar 

  19. Nicholson GL, Blaustein J, Etzler ME: Characterization of two plant lectins from Ricinus communis and their quantitative interaction with a murine lymphoma. Biochemistry 13: 196–204 (1974).

    Google Scholar 

  20. Olsnes S, Pihl A: In: Cohen P, vanHeyningen S (eds) Molecular Actions of Toxins and Viruses, pp. 51–105. New York: Elsevier Biomedical Press (1982).

    Google Scholar 

  21. Olsnes S, Refsnes K, Christiansen TB, Pihl A: Studies on the structure and properties of the lectins from Abrus precatorius and Ricinus communis. Biochim Biophys Acta 405: 1–10 (1975).

    Google Scholar 

  22. Olsnes S, Refsnes K, Pihl A: Mechanism of action of the toxic lectins abrin and ricin. Nature 249: 627–631 (1974).

    Google Scholar 

  23. Roberts LM, Lamb FI, Pappin DJC, Lord JM: The primary sequence of Ricinus communis agglutinin. Comparison with ricin. J Biol Chem 260: 15682–15686 (1985).

    Google Scholar 

  24. Robertus JD, Ready MP: Ricin B chain and discoidin share a common primitive protein fold. J Biol Chem 259: 13953–13956 (1984).

    Google Scholar 

  25. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci 74: 5463–5467 (1977).

    Google Scholar 

  26. Sperti S, Montanaro L, Mattiola A, Stirpe E: Inhibition by ricin of protein synthesis in vitro: 60S ribosomal subunit as the target of the toxin. Biochem J 136: 813–815 (1973).

    Google Scholar 

  27. Villafranca JE, Robertus JD: Ricin B chain is a product of gene duplication. J Biol Chem 256: 554–556 (1981).

    Google Scholar 

  28. Wallace RB, Johnson MJ, Hirose T, Miyake T, Kawashima EH, Itakura K: The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit β-globin. Nucleic Acids Res 9: 879–894 (1981).

    Google Scholar 

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Ladin, B.F., Murray, E.E., Halling, A.C. et al. Characterization of a cDNA encoding ricin E, a hybrid ricin-Ricinus communis agglutinin gene from the castor plant Ricinus communis . Plant Mol Biol 9, 287–295 (1987). https://doi.org/10.1007/BF00166464

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