Abstract
Frankia is the diverse bacterial genus that fixes nitrogen within root nodules of actinorhizal trees and shrubs. Systematic and ecological studies of Frankia have been hindered by the lack of morphological, biochemical, or other markers to readily distinguish strains. Recently, nucleotide sequence of 16 S RNA from the small ribosomal subunit has been used to classify and identify a variety of microorganisms. We report nucleotide sequences from portions of the 16 S ribosomal RNA from Frankia strains AcnI1 isolated from Alnus viridis ssp. crispa (Ait.) Turrill and PtI1 isolated from Purshia tridentata (Pursh) DC. The number of nucleotide base substitutions and gaps we find more than doubles the previously reported sequence diversity for the same variable regions within other strains of Frankia.
This is a preview of subscription content, log in via an institution to check access.
References
Baker, D 1987 Physiol. Plant. 70, 245–248.
Benson, D R 1988 Microbiol. Sci. 5, 9–12.
Dawson, J O 1986 Outl. Agric. 15, 202–208.
Dawson, J O et al. 1989 Plant and Soil 118, 1–11.
Embley, T M et al. 1988 J. Gen. Microbiol. 134, 961–966.
Gardes, M and Lalonde, M 1987 Physiol. Plant 70, 237–244.
Göebel, U B et al. 1987 J. Gen. Microbiol. 133, 1969–1974.
Hahn, D et al. 1989a Plant and Soil 118, 211–219.
Hahn, D et al. 1989b System. Appl. Microbiol. 11, 236–242.
Hahn, D et al. 1990 Appl. Environ. Microbiol. 56, 1342–1346.
Lalonde, M and Calvert, H E 1979 In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. J CGordon, C TWheeler, and D APerry. pp 95–110. Oregon State University, Corvallis, OR.
Lalonde, M et al. 1988 In Nitrogen Fixation, Hundred Years After. Eds. H OBothe, F JdeBruijn and W ENewton. pp 671–680. Proc. 7th Internatl. Congr. Triple-bond Nitrogen Fixation, Köln, FRG., March 15–20, 1988 Gustav Fischer, Stuttgart.
Lane, D J et al. 1985 Proc. Natl. Acad. Sci. USA 82, 6955–6959.
Lane, D J et al. 1988 Methods Enzymol. 167, 138–144.
Olsen, G J et al. 1986 Annu. Rev. Microbiol. 40, 337–365.
Pace, N R et al. 1986 Adv. Microbiol. Ecol. 9, 1–55.
Reddell, P et al. 1988 Plant and Soil 108, 79–86.
Sanger, T et al. 1977 Proc. Natl. Acad. Sci. USA 74, 5463–5467.
Sougoufara, B et al. 1989 Plant and Soil 118, 133–138.
Woese, C R 1987 Microbiol. Rev. 51, 221–271.
Yang, D et al. 1985 Proc. Natl. Acad. Sci. USA 82, 4443–4447.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Harry, D.E., Yang, D.C. & Dawson, J.O. Nucleotide sequence and diversity in 16S ribosomal RNA from Frankia . Plant Soil 131, 143–146 (1991). https://doi.org/10.1007/BF00010429
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00010429