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Physiological effects of photosystem II-herbicides on the development of the photosynthetic apparatus

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Abstract

Photosystem II-herbicides (bentazone*, diuron) not only block photosynthetic electron transport, but have additional effects on the cell metabolism of Raphanus seedlings. They induce the formation of shade-type chloroplasts with a different ultrastructure and prenyllipid composition. This is shown by higher grana stacks as well as by higher chlorophyll b and lutein amounts with reference to chlorophyll a, and lower levels of the plastidic prenylquinones (plastoquinone, phylloquinone, α-tocoquinone) and β-carotene as compared to the controls.

The two herbicides bentazone and diuron change the labelling pattern of the chloroplast pigments from 3H-mevalonic acid and 2-14C-acetate and also reduce the accumulation of anthocyanin (pelargonidin), which is a further indication of a shade-type growth response. The level of ubiquinone, an indicator for mitochondrial activity, is, however, increased.

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References

  1. Abel, B (1956) Ober die Beerinflussung der Chloroplastenstruktur durch Licht bei Antirrhinum majus (haploid). Naturwissenschaften 43: 136–137.

    Google Scholar 

  2. Ashton, FM and Crafts, AS (1973) Mode of action of herbicides. New York: J. Wiley.

    Google Scholar 

  3. Audus, LJ (1976) Herbicides, Vol. 1, p. 608. New York: Academic Press.

    Google Scholar 

  4. Boardman, NK, Björkman, O, Anderson, JM, Goodchild, DJ and Thorne, SW (1974) Photosynthetic adaptation of higher plants to light intensity: relationship between chloroplast structure, composition of the photosystems and photosynthetic rates. Proc 3rd Int Congr on Photosynthesis, Avron, M ed., Vol. III, pp 1809–1827. Amsterdam: Elsevier.

    Google Scholar 

  5. Britton, G and Goodwin, JW (1971) Biosynthesis of carotenoids. Methods in enzymology XVIII, McCormick, PB and Wright, LD, eds., pp 654–701. New York: Academic Press.

    Google Scholar 

  6. Corbett, JR (1974) The biochemical mode of action of pesticides. New York: Academic Press.

    Google Scholar 

  7. Fedtke, C (1974) Auslösung von Schattenanpassungsreaktionen in Weizenpflanzen durch den Photosynthesehemmstoff N-(2-Benzthiazolyl)-N,N′-dimethylharnstoff. Ber Dtsch Bot Ges 87: 155–160.

    Google Scholar 

  8. Fedtke, C (1979) Plant physiological adaptations induced by low rates of photosynthesis. Z Naturforsch 34c: 932–935.

    Google Scholar 

  9. Fedtke, C, Deichgräber, G and Schnepf, E (1977) Herbicide induced changes in wheat chloroplast ultrastructure and chlorophyll a/b ratio. Biochem Physiol Pflanz 171: 307–312.

    Google Scholar 

  10. Hager, A and Meyer-Bertenrath, J (1966) Verteilungscgromatographische Trennung von Chlorophyllen und Carotinoiden grüner Pflanzen an Dünnschichten. Planta 58: 546–568.

    Google Scholar 

  11. Hager, A and Meyer-Bertenrath, J (1967) Die Isolierung und qualtitative bestimmung der Carotinoide und Chlorophylle von Blättern, Algen und isolierten Chloroplasten mit Hilfe dünnschichtchromatographischer Methoden. Planta 69: 198–217.

    Google Scholar 

  12. Klein, S and Neumann, J (1966) The greening of etiolated bean leaves and the development of chloroplast fine structure in absence of photosynthesis. Plant Cell Physiol 7: 115–123.

    Google Scholar 

  13. Kleudgen, HK (1978) The influence of the herbicide methabenzthiazuron on the synthesis of plastidic lipids in Hordeum vulgare seedlings. Biochem Physiol Pflanz 9: 57–60.

    Google Scholar 

  14. Köhler K-H (1977) Interactions between cytokinins, gibberellins and light in the regulation of amaranthin synthesis. Abstr Conf on Regulation of Developmental Processes in Plants, Halle, p. 216.

  15. Lichtenthaler HK (1969) Localization and functional concentration of lipoquinones in chloroplasts. In Metzner H, ed. Progress in photosynthesis research, Vol. 1, pp 304–314.

  16. Lichtenthaler, HK (1977) Regulation of prenylquinone synthesis in higher plants. In Tevini, M and Lichtenthaler, HK, eds. Lipids and lipid polymers in higher plants, pp 231–258. Berlin: Springer.

    Google Scholar 

  17. Lichtenthaler, HK (1979) Occurrence and function of prenyllipids in the photosynthetic membrane. In Appelqvist, LA and Liljenberg, C, eds. Advances in the biochemistry and physiology of plant lipids, pp 57–58. Amsterdam: Elsevier.

    Google Scholar 

  18. Lichtenthaler, HK (1979) Effect of biocides on the development of the photosynthetic apparatus of radish seedlings grown under strong and weak light conditions. Z Naturforsch 34c: 936–940.

    Google Scholar 

  19. Lichtenthaler, HK and Prenzel, U (1977) High-performance liquid chromatography of natural prenylquinones. J Chromatogr 135: 493–498.

    Google Scholar 

  20. Lichtenthaler, HK, Karunen, P and Grumbach, KH (1977) Determination of prenylquinones in green photosynthetic active moss and liver moss tissues. Physiol Plant 40: 105–110.

    Google Scholar 

  21. Lichtenthaler, HK and Buschmann, C (1978) Control of chloroplast development by red light, blue light and phytohormones. In Akoyunoglou, G et al, eds. Chloroplast development, pp 801–816. Amsterdam: Elsevier/North Holland Biomedical Press.

    Google Scholar 

  22. Nobel, P (1967) Light-induced chloroplast shrinkage in vivo detectable after rapid isolation of chloroplasts from Pisum sativum. Plant Physiol 42: 1389–1394.

    Google Scholar 

  23. Palade, GE (1952) A study of fixation for electron microscopy. J Ex Med 95: 285–297.

    Google Scholar 

  24. Pfister, K, Buschmann, C and Lichtenthaler, HK (1974) Inhibition of the photosynthetic electron transport by bentazone. In Avron, M, ed. Proc Third Int Cong on Photosynthesis, Vol. I, pp 675–681. Amsterdam: Elsevier.

    Google Scholar 

  25. Pfister, K and Arntzen, CJ (1979) The mode of action of photosystem II-specific inhibitors in herbicide-resistant weed biotypes. Z Naturforsch 34c: 996–1009.

    Google Scholar 

  26. Penger, G (1979) Studies about the mechanism of herbicidal interaction with photosystem II in isolated chloroplasts. Z Naturforsch 34c: 1010–1014.

    Google Scholar 

  27. Retzlaff, G, Hilton, JL and John, GBSt (1979) inhibition of photosynthesis by bentazone in intact plants and isolated cells in relation to the pH. Z. Naturforsch 34c: 994–997.

    Google Scholar 

  28. Reynolds, ES (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17: 208–212.

    Google Scholar 

  29. Siefermann-Harms, D and Ninnemann, H (1979) The separation of photochemically active PS 1 and PS 2 containing chlorophyll-protein complexes by iso-electric focusing of bean thylakoids on polyacrylamide gel plates. FEBS Lett 104: 71–77.

    Google Scholar 

  30. Spurr, AR (1969) A low-viscosity expoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26: 31–43.

    Google Scholar 

  31. Straub, V and Lichtenthaler, HK (1973) Die Wirkung von Gibberellinsäure A3 und Kinetin auf die Bildung der Photosynthesepigmente, Lipochinone und Anthocyane in Raphanus-Keimlingen. Z Pflanzenphyiol 70: 308–321.

    Google Scholar 

  32. Thornber, JP (1975) Chlorophyll-proteins: light-harvesting and reaction center components of plants. Annu Rev Plant Physiol 26: 127–158.

    Google Scholar 

  33. Tischer, W and Strotmann, H (1979) Some properties of the DCMU-binding site in chloroplasts. Z Naturforsch 34c: 992–995.

    Google Scholar 

  34. Trebst, A (1979) Inhibition of photosynthetic electron flow by phenol and diphenylether herbicides in control and trypsin-treated chloroplasts. Z Naturforsch 34c: 986–991.

    Google Scholar 

  35. Trebst, A and Harth, E (1974) N-alkylated-ureas and ringclosed N-acylamides as inhibitors of photosystem II. Z Naturforsch 29c: 232–235.

    Google Scholar 

  36. Verbeek, L and Lichtenthaler, HK (1973) Der Einfluß von Stickstoffmangel auf die Lipochinon- und Isoprenoidsynthese der Chloroplasten von Hordeum vulgare L. Z Pflanzenphysiol 70: 245–258.

    Google Scholar 

  37. Wessels, JSC and van derVeen, R (1956) The action of some derivatives of phenyl-urethan and of 3-phenyl-1,1-dimethylurea on the Hill reaction. Biochim Biophys Acta 19: 548–549.

    Google Scholar 

  38. Wild, AKeB and Shano, ER (1973) The effect of light intensity during growth of Sinapis alba on the electron-transport components. Z Pflanzenphysiol 69: 334–350.

    Google Scholar 

  39. Wild, A, Rühle, W and Grahl, H (1975) The effect of light intensity during growth of Sinapis alba on the electron-transport and the noncyclic photophosphorylation. In Marcelle, R, ed. Environmental and biological control of photosynthesis, pp 115–121. The Hague: Dr W Junk.

    Google Scholar 

  40. Ziegler, R and Egle, K (1965) Zur quantitativen Analyse der Chloroplastenpigmente, I: Kritische Überprüfund der spektralphotometrischen Chlorophyllbestimmung. Beitr Biol Pflanz 41: 11–37.

    Google Scholar 

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Authors and Affiliations

  1. Botanical Institute, University of Karlsruhe, D-7500, Karlsruhe, Federal Republic of Germany

    H. K. Lichtenthaler, G. Burkard, K. H. Grumbach & D. Meier

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  1. H. K. Lichtenthaler
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  2. G. Burkard
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  3. K. H. Grumbach
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  4. D. Meier
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Lichtenthaler, H.K., Burkard, G., Grumbach, K.H. et al. Physiological effects of photosystem II-herbicides on the development of the photosynthetic apparatus. Photosynth Res 1, 29–43 (1980). https://doi.org/10.1007/BF00020073

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  • DOI: https://doi.org/10.1007/BF00020073

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