Abstract
The protolytic reactions of PSII membrane fragments were analyzed by measurements of absorption changes of the water soluble indicator dye bromocresol purple induced by a train of 10 μs flashes in dark-adapted samples. It was found that: a) in the first flash a rapid H+-release takes place followed by a slower H+-uptake. The deprotonation is insensitive to DCMU but is completely eliminated by linolenic acid treatment of the samples; b) the extent of the H+-uptake in the first flash depends on the redox potential of the suspension. In this time domain no H+-uptake is observed in the subsequent flashes; c) the extent of the H+-release as a function of the flash number in the sequence exhibits a characteristic oscillation pattern. Multiphasic release kinetics are observed. The oscillation pattern can be satisfactorily described by a 1, 0, 1, 2 stoichiometry for the redox transitions Si → Si+1 (i=0, 1, 2, 3) in the water oxidizing enzyme system Y. The H+-uptake after the first flash is assumed to be a consequence of the very fast reduction of oxidized Q400(Fe3+) formed due to dark incubation with K3[Fe(CN)6]. The possible participation of component Z in the deprotonation reactions at the PSII donor side is discussed.
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Abbreviations
- A:
-
protonizable group at the PSII acceptor side
- BCP:
-
Bromocresol Purple
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- FWHM:
-
Full Width at Half Maximum
- QA, QB :
-
primary and secondary plastoquinone at PSII acceptor side
- Q400 :
-
redox group at PSII-acceptor side (high spin Fe2+)
- P680:
-
Photoactive chlorophyll of PSII reaction center
- Si :
-
redox states of the catalytic site of water oxidation
- Z:
-
redox component connecting the catalytic site of water oxidation with the reaction center
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Renger, G., Wacker, U. & Völker, M. Studies on the protolytic reactions coupled with water cleavage in photosystem II membrane fragments from spinach. Photosynth Res 13, 167–184 (1987). https://doi.org/10.1007/BF00035239
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DOI: https://doi.org/10.1007/BF00035239