Abstract
The inhibitory effect of Zn2+ on photosynthetic electron transport was investigated in native and CaCl2-treated (depleted in extrinsic polypeptides) Photosystem II (PS II) submembrane preparations. Inhibition of 2,6-dichlorophenolindophenol photoreduction by Zn2+ was much stronger in protein-depleted preparations in comparison to the native form. It was found that Ca2+ significantly reduced the inhibition in the native PS II preparations, as did Mn2+ in a combination with H2O2 in the protein-depleted counterparts. No other tested monovalent or divalent cations could replace Ca2+ or Mn2+ in the respective experiments. Diphenylcarbazide could partially relieve (40–45%) the inhibition in both types of preparations. The above indicates the presence of an active Zn2+ inhibitory site on the donor side of PS II. However, neither Ca2+ nor Mn2+ could completely prevent inhibition by high concentrations of Zn2+ (>1 mM). We propose that elevated levels of Zn2+ strongly perturb the conformation of the PS II core complex and might also affect the acceptor side of the photosystem.
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Abbreviations
- PMSF:
-
phenylmethanesulfonyl fluoride
- MES:
-
2-(N-morpholino)ethane sulphonic acid
- Chl:
-
chlorophyll
- PS II:
-
Photosystem II
- DCIP:
-
2,6-dichlorophenolindophenol
- DPC:
-
sym-diphenylcabazide
- DCBQ:
-
2,5-dichlorobenzoquinone
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Rashid, A., Bernier, M., Pazdernick, L. et al. Interaction of Zn2+ with the donor side of Photosystem II. Photosynth Res 30, 123–130 (1991). https://doi.org/10.1007/BF00042010
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DOI: https://doi.org/10.1007/BF00042010