Abstract
Usisng intact leaves, the extent of the decrease in chlorophyll a fluorescenece caused by the addition of continuous 710 nm light superimposed on modulated (20 Hz) 550 nm light was used to determine the distribution of this absorbed light between photosystems I (α) and II (β). The Fo and Fm levels, which defined the total variable fluorescenece, were taken as equal to those obtained with excess 710 nm light and with saturating blue-green light, respectively.
An analogous procedure was used with a photoacoustic detector, saturating white light defining a base line for oxygen yield, the levels with an without 710 nm light being used to define β and α respectively.
The two methods gave similar values for the distribution of light between the two photosystems for the experimental conditions used, β averaging 0.55 for a range of Triticum genotypes and Brachypodium sylvaticum grown in high or low light.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Austin RB, Morgan CL, Ford MA and Bhagwat SG (1982) Flagleaf photosynthesis of Triticum aestivum and related diploid and tetraploid species. Ann Bot 49: 177–189
Bults G, Horwitz BA, Malkin S and Cahen D (1982) Photoacoustic measurements of photosynthetic activities in whole leaves — photochemistry and gas exchange. Biochim Biophys Acta 679: 452–465
Bunce JA, Patterson DT, Peet MM, Alberte RS (1977) Light acclimation during and after leaf expansion in Soybean. Plant Physiol 60: 255–258
Canaani O and Malkin S (1984) Distribution of light excitation in an intact leaf between the two photosystems of photosynthesis — changes of absorption following state 1–state 2 transitions. Biochim Biophys Acta 766: 513–524
Canaani O and Malkin S (1984) Physiological adaptation to a newly observed low light intensity state in intact leaves, resulting in extreme imbalance in excitation energy distribution between the two photosystems. Biochim Biophys Acta 766: 525–532
Chow WS, Telfer A, Chapman DJ and Barber J (1981) State 1–state 2 transition in leaves and its association with ATP induced chlorophyll fluorescence quenching. Biochim Biophys Acta 638: 60–68
Clough JM, Alberte RS and Teevi JA (1979) Photosynthetic adaptation of Solanum dulcamara L. to sun and shade environments. II Physiological characterisation of phenotypic response to environment. Plant Physiol 64: 25–30
Freeman TP, Duysen ME, Olson NH and Williams ND (1982) Electron transport and chloroplast ultrastructure of a chlorophyll deficient mutant of wheat. Phot Res 3: 179–189
Furbank RT, Bagder MR and Osmond CB (1983) Photoreduction of oxygen in mesophyll chloroplasts on C4 plants — a model system for studying an in vivo Mehler reaction. Plant Physiol 73: 1038–1041
Harvey GW (1980) Seasonal alteration of photosynthetic unit sizes in three herb layer components of a deciduous forest community. Amer J Bot 67: 293–299
Kanstad SO, Cahen D and Malkin S (1983) Simultaneous detection of photosynthetic energy storage and oxygen evolution in leaves by photothermal radiometry and photoacoustics. Biochim Biophys Acta 722: 182–189
Kok B and Cheniae G (1966) Kinetics and intermediates of the oxygen evolution steps in photosynthesis. In Sanadi R, ed. Current topics in Bioenergetics Vol I pp 1–47. Academic Press New York
Kok B, Malkin S, Owens O and Forbush B (1966) Observations on the reducing side of the O2-evolving photoact. In Brookhaven Symp Biology 19, pp446–459. Associated Univ Inc — Brookhaven Natl Lab USA
Krause GH (1973) The high energy state of the thylakoid system as indicated by chlorophyll fluorescence and chloroplast shrinkage. Biochim Biophys Acta 292: 715–728
Krause GH, Briantais JM and Vernotte C (1983) Characterization of chlorphyll fluorescence quenching in chloroplasts by flurescence spectroscopy at 77°K. Biochim Biophys Acta 723: 169–175
Lasser-Ross N, Malkin S and Cahen D (1980) Photoacoustic detection of photosynthetic activities in isolated broken chloroplasts. Biochim Biophys Acta 593: 330–341
Lavorel J and Etienne AL (1977) In vivo chlorophyll fluorescence. In: Barber J (ed) Topics in Photosynthesis Vol 2 Primary Process of Photosynthesis, pp 203–268. Elsevier Amsterdam, New York, Oxford
Leong T-Y and Anderson JM (1984) Adaptation of the thylakoid membranes of pea chloroplasts to light intensities. II. Regulation of electron transport capacities, electron carriers, coupling factor (CF1) activity and rates of photosynthesis. Phot Res 5: 117–128
Malkin S, Armond P, Mooney H and Fork D (1981) Photosystem II Photosynthetic Unit sizes from fluorescence induction in leaves — correlation to photosynthetic capacity. Plant Physiol 67: 570–579
Malkin S and Cahen D (1979) Photoacoustic spectroscopy and radiant energy conversion: Theory of the effect with special emphasis on photosynthesis. Photochem Photobiol 29: 803–813
Malkin S and Fork DC (1981) Photosynthetic units of sun and shade plants. Plant Physiol 67: 580–583
Malkin S and Kok B (1966) Fluorescence induction studies in isolated chloroplasts. I. Number of components involved in the reaction and quantum yields. Biochim Biophys Acta 126: 413–422
Morgan CL and Austin RB (1986) Analysis and fluorescence transients of DCMU-treated leaves of Triticum species to provide estimates of the densities of photo-system II reaction centres. Phot Res 7: 203–219
Poulet P, Cahen D and Malkin S (1983) Photoacoustic detection of photosynthetic oxygen evolution from leaves. Quantitative analysis by phase and amplitude measurements. Biochim Biophys Acta 724: 433–446
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Malkin, S., Morgan, C.L. & Austin, R.B. Estimation of the light distribution between photosystems I and II in intact wheat leaves by fluorescence and photoacoustic measurements. Photosynth Res 7, 257–267 (1986). https://doi.org/10.1007/BF00014679
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00014679