Abstract
Cell suspension cultures of Lithospermum erythrorhizon, Gardenia jasminoides and Nicotiana tabacum were capable of glucosylating esculetin to esculin (7-hydroxycoumarin-6-O-β-D-glucoside). Especially, a culture strain of Lithospermum erythrorhizon was superior in the esculetin glucosylating capability; 40 to 50% of esculetin administered to the culture medium at early stationary growth stage was converted into esculin within 24 h. The rate of glucosylation was also dependent on the growth stage and the medium composition especially growth hormones and sugar.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barz W, Ellis BE (1981) Plant cell cultures and their biotechnological potential. Ber Deutsh Bot Ges Bd 94:1–26
Döller PC, Alfermann AW, Reinhard E (1977) Biotransformation of cardenolides by cell suspension cultures of Digitalis lanata and Thevetia neriifolia. Plant Med 31:1–6
Furuya T (1978) Biotransformation by plant cell cultures. In: Thorpe TA (ed.) Fronties of plant tissue culture. Calgary, Canada: University of Calgary pp 191–200
Heins M (1978) Screening of Digitalis lanata plants and cell cultures for hydroxylation capacity. In: Alfermann AW, Reinhard E (eds.) Production of natural compounds by cell culture methods. München: University of Tübingen pp 39–47
Ibrahim R, Boulay B (1980) Purification and some properties of UDP-glucose: o-dihydroxycoumarin 7–0-glucosyltransferase from tobacco cell cultures. Plant Science Letters 18:177–184
Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100–127
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Okazaki M, Hino F, Kominami K, Miura Y (1982) Effects of plant hormones on formation of scopoletin and scopolin in tobacco tissue cultures. Agric Biol Chem 46:609–614
Pilgrim H (1970) Untersuchungen zur glykosidbindung in pflanzlichen Gewebekulturten. Pharmazie 25:568
Reinhard E (1974) Biotransformation by plant tissue cultures. In: Street HE (ed.) Tissue culture and plant science. London: Academic press pp 433–459
Reinhard E, Alfermann AW (1980) Biotransformation by plant tissue cultures. In: Fiechter A (ed.) Advances in biochemical engineering. Berlin: Springer-Verlag pp 49–83
Sargent JA, Skoog F (1960) Effects of indoleacetic acid and kinetin on scopoletinscopolin levels in relation to growth of tobacco tissues in vitro. Plant Physiol. 35: 934–941
Sato M, Hasegawa M (1972) Transglucosylases in Cichorium intybus converting cichoriin to esculin. Phytochem. 11:3149–3156
Skoog F, Montaldi E (1961) Auxin-kinetin interaction regulating the scopoletin and scopolin levels in tobacco tissue cultures. Proc. Nat. Acad. Sci. (USA) 47:36–49
Tabata M, Ikeda F, Hiraoka N, Konoshima M (1976) Glucosylation of phenolic compounds by Datura innoxia suspension cultures. Phytochem 15:1225–1229
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tabata, M., Umetani, Y., Shima, K. et al. Glucosylation of esculetin by plant cell suspension cultures. Plant Cell Tiss Organ Cult 3, 3–9 (1984). https://doi.org/10.1007/BF00035916
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00035916