Abstract
Segments of young inflorescences of Triticum aestivum cv. Chinese Spring (CS), its F1 hybrids with Agropyron trachycaulum and A. scirpeum and backcross derivatives with A. yezoense, A. intermedium and A. junceum, and of a A. yezoense x T. aestivum cv. Wichita hybrid were cultured. Different parts of young spikelets of A. trachycaulum x CS F1 and A. yezoense x Wichita F1 's were also cultured. Percent callus induction was lower in wheat than in the wheat-Agropyron hybrids or backcross derivatives. Percent callus induction from different organs in both hybrids was in the descending order of whole spikelet, spikelet without glumes, rachis, and glumes. No plants could be regenerated from calli of wheat and backcross derivatives except those of CS x A. intermedium combination. Callus induction in hybrids varied from 54 to 84% and plant regeneration from 14 to 31%. The regenerants required no vernalization. Variants including one with top-dense spikes and another with elongated spikelets were recovered. Out of eight A. trachycaulm x CS hybrid regenerants, one had anthers and stigma as opposed to neutral flowers of the original hybrid.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brettell RIS, Wernicke W, Thomas E (1980) Embryogenesis from cultured immature inflorescences of Sorghum bicolor. Protoplasma 104:141–148
Chin JC, Scott KJ (1977) Studies on the formation of roots and shoots in wheat callus culture. Ann Bot 41:473–481
Gosch-Wackerle G, Avivi L, Galun E (1979) Induction, culture and differentiation of callus from immature rachises, seeds and embryos of Triticum. Z Pflanzenphysiol 91:267–278
Heinz DJ, Mee GWP (1969) Plant differentiation from callus tissue of Saccharum species. Crop Sci 9:346–348
Kihara H, Tsunewaki K (1961) Pistillody of Triticum durum induced by alien cytoplasm. Seiken Zihô 12:1–10
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nakamura C, Keller WA (1982) Plant regeneration of inflorescence cultures of hexaploid triticale. PI Sci Lett 24:275–280
Nakamura C, Keller WA, Fedak G (1981) In vitro propagation and chromosome doubling of a Triticum crassum and Hordeum vulgare intergeneric hybrid. Theor Appl Genet 60:89–96
Ozias-Akins P. Vasil IK (1982) Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat): Evidence for somatic embryogenesis. Protoplasma 110:95–105
Rice TB, Reid RK, Gordon PN (1978) Morphogenesis in field crops. In: Hughes KW, Henke R, Constantin M (eds.) Propagation of higher plants through tissue culture: A bridge between research and application. U.S. Dept. of Energy (Conf. 7804111), Washington, DC, pp. 262–267
Sharma HC, Gill BS (1983) New hybrids between Agropyron and wheat. 2. Production, morphology and cytogenetic analysis of F1 hybrids and backcross derivatives. Theor Appl Genet 66:111–121
Sears RG, Deckard EL (1982) Tissue culture variability in wheat: Callus induction and plant regeneration. Crop Sci 22:546–550
Tsitsin NV, Lubimova VF (1959) New species and forms of cereals derived from hybridization between wheat and couchgrass. Am Nat 93:181–191
Vasil V, Vasil IK (1981) Somatic embryogenesis and plant regeneration from tissue cultures of Pennisetum americanum, and P. americanum x P. purpureum hybrid. Am J Bot 68:864–872
Wang D-Y, Vasil IK (1982) Somatic embryogenesis and plant regeneration from inflorescence segments of Pennisetum purpureum Schum. (Napier or Elephant grass). Plant Sci Lett 25:147–154
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sharma, H.C., Gill, B.S. & Sears, R.G. Inflorescence culture of wheat-Agropyron hybrids: Callus induction, plant regeneration, and potential in overcoming sterility barriers. Plant Cell Tiss Organ Cult 3, 247–255 (1984). https://doi.org/10.1007/BF00040344
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00040344