Abstract
This communication reviews data on the accumulation and biosynthesis of rosmarinic acid in cell suspension cultures ofColeus blumei. The influence of the medium, mainly the carbohydrate source on growth and rosmarinic acid production in these cell cultures is described. The biosynthetic pathway of rosmarinic acid was elucidated inColeus blumei cell cultures: eight enzymatic activities are involved in the transformation of the precursors phenylalanine and tyrosine to the end product rosmarinic acid.
Similar content being viewed by others
Abbreviations
- CAH:
-
cinnamic acid 4-hydroxylase
- 4CL:
-
4-coumarate:CoA ligase
- HPPR:
-
hydroxyphenylpyruvate reductase
- 3-H:
-
hydroxycinnamoyl-hydroxyphenyllactate 3-hydroxylase
- 3′-H:
-
hydroxycinnamoyl-hydroxyphenyllactate 3′-hydroxylase
- PAL:
-
phenylalanine ammonia-lyase
- RAS:
-
rosmarinic acid synthase (hydroxycinnamoyl-CoA:hydroxyphenyllactate hydroxycinnamoyl transferase)
- TAT:
-
tyrosine aminotransferase
References
Chaprin N & Ellis BE (1984) Microspectrophotometric evaluation of rosmarinic acid accumulation in single cultured plant cells. Can. J. Bot. 62: 2278–2282
De-Eknamkul W & Ellis BE (1987) Tyrosine aminotransferase: the entrypoint enzyme of the tyrosine-derived pathway in rosmarinic acid biosynthesis. Phytochemistry 26: 1941–1946
De Tommasi N, De Simone F, De Feo V & Pizza C (1991) Phenylpropanoid glycosides and rosmarinic acid fromMomordica balsamina. Planta Medica 57: 201
Ellis BE & Towers GHN (1970) Biogenesis of rosmarinic acid inMentha. Biochem. J. 118: 291–297
Gertlowski C (1991) Einfluß des Kulturmediums auf die Produktion der Rosmarinsäure in Suspensionskulturen vonColeus blumei. Diploma Thesis, University of Düsseldorf
Gertlowski C & Petersen M (1993) Influence of the carbon source on growth and rosmarinic acid production in suspension cultures ofColeus blumei. Plant Cell Tiss. Org. Cult. 34: 183–190
Harborne JB (1966) Caffeic acid ester distribution in higher plants. Z. Naturforsch. 21b: 604–605
Häusler E, Petersen M & Alfermann AW (1991) Hydroxyphenylpyruvate reductase from cell suspension cultures ofColeus blumei Benth. Z. Naturforsch. 46c: 371–376
Häusler E, Petersen M & Alfermann AW (1992) Rosmarinsäure inBlechnum-Spezies. In: Haschke HP & Schnarrenberger C (Eds) Botanikertagung 1992 Berlin (p 507). Akademie Verlag, Berlin
Häusler E, Petersen M & Alfermann AW (1993) Isolation of protoplasts and vacuoles from cell suspension cultures ofColeus blumei Benth. Plant Cell Rep. 12: 510–512
Hiller K & Kothe N (1967) Chlorogen- und Rosmarinsäure — Vorkommen und quantitative Verteilung in Pflanzen der Saniculoideae. Pharmazie 22: 220–221
Kempin B (1994) Untersuchungen zur Rosmarinsäuresynthese in Suspensionskulturen vonColeus blumei: Tyrosin Aminotransferase. Diploma Thesis, University of Düsseldorf
Lamaison JL, Petitjean-Freytet C & Carnat A (1990) Teneurs en acide rosmarinique, en dérivés hydroxycinnamiques totaux et activité antioxydante chez les Apiacées, les Borraginacées et les Lamiacées médicinales. Ann. pharmaceutiques françaises 48: 103–108
Mölgaard P & Ravn H (1988) Evolutionary aspects of caffeoyl ester distribution in dicotyledons. Phytochemistry 27: 2411–2421
Petersen MS (1991) Characterization of rosmarinic acid synthase from cell cultures ofColeus blumei. Phytochemistry 30: 2877–2881
Petersen M & Alfermann AW (1988) Two new enzymes of rosmarinic acid biosynthesis from cell cultures ofColeus blumei: Hydroxyphenylpyruvate reductase and rosmarinic acid synthase. Z. Naturforsch. 43c: 501–504
Petersen M, Häusler E, Karwatzki B & Meinhard J (1993) Proposed biosynthetic pathway for rosmarinic acid in cell cultures ofColeus blumei Benth. Planta 189: 10–14
Razzaque A & Ellis BE (1977) Rosmarinic acid production inColeus cell cultures. Planta 137: 287–291
Scarpati ML & Oriente G (1959) Isolamento e costituzione dell'acido rosmarinico (dalrosmarinus off.). Ric. Sci. 28: 2329–2333
Takeda R, Hasegawa J & Sinozaki K (1990) Phenolic compounds from Anthocerotae. In: Zinsmeister HD & Mues R (Eds) Bryophytes. Their Chemistry and Chemical Taxonomy (pp 201–207). Oxford Science Publications, Oxford
Zenk MH, El-Shagi H & Ulbrich B (1977) Production of rosmarinic acid by cell-suspension cultures ofColeus blumei. Naturwissenschaften 64: 585–586
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Petersen, M., Szabo, E., Meinhard, J. et al. Biosynthesis and accumulation of rosmarinic acid in suspension cultures ofColeus blumei . Plant Cell Tiss Organ Cult 43, 89–92 (1995). https://doi.org/10.1007/BF00052161
Issue Date:
DOI: https://doi.org/10.1007/BF00052161