Skip to main content
SpringerLink
Log in

Natural product formation by plant cell biotechnology

Results and perspectives

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

This short review tries to compile some results, which show the importance and the potential of plant cell and tissue cultures for a biotechnological production of natural products. On the other hand, it can not be denied, that a real breakthrough of this technique has not yet been achieved. The problems involved and possible ways to overcome these will be discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Akiu S, Suzuki Y, Fujinuma Y, Asahara T & Fukuda M (1988) Inhibitory effect of arbutin on melanogenesis: Biochemical study in cultured B16 melanoma cells and effect on the UV-induced pigmentation in human skin. Proc. Japan. Soc. Invest. Dermatol. 12, 138–139

    Google Scholar 

  • Alfermann AW, Bergmann W, Figur C, helmbold U, Schwantag D, Schuller I & Reinhard E (1983) Biotransformation of β-methyldigitoxin to β-methyldigoxin by cell cultures ofDigitalis lanata. In: Mantell SH & Smith H (Eds) Plant Biotechnology (pp 67–74). Cambridge University Press, Cambridge, UK

    Google Scholar 

  • Allison AJ, Butcher DN, Connolly JD & Overton KH (1968) Paniculides A, B, and C, bisabonolenoid lactones from tissue cultures ofAndrographis paniculata. Chem. Comm. 1493

  • Ball E (1967) Production of a group of anthocyanins in a callus culture under the influence of an auxin. Plant Physiol. 42 Suppl. S24

  • Berlin J, Wray V, Mollenschott C & Sasse F (1986) Formation of β-peltatin-A-methylether and coniferin by root cultures ofLinum flavum. J. Nat. Prod. 49:435–439

    Google Scholar 

  • Broomhead AJ & Dewick PM (1990) Aryltetralin lignans fromLinum flavum andLinum capitatum. Phytochem 29:3839–3844

    Google Scholar 

  • DeCapite L (1955) Azione degli zuccheri e delle basse temperature sulla formatione degli antociani in radici diDaucus carota coltivato in vitro. Ric. Sci. 35: 2091–2097

    Google Scholar 

  • Eilert UFK, Kurz WGW & Constabel F (1985) Stimulation of sanguinarine accumulation inPapaver somniferum cell cultures by fungal elicitors. J Plant Physiol. 119:65–76

    Google Scholar 

  • Farnsworth NR (1985) The role of medicinal plants in plants in drug development. In: Kroogsgard-Larsen P, Brogger Christensen S. & Koford H (Eds) Natural Products and Drug Development (pp 17–30) Munksgaard Copenhagen, Denmark

    Google Scholar 

  • Fujita Y, Tabata M, Nishi A & Yamada Y (1982) New medium and production of secondary compounds with the two-staged culture method. In: Fujiwara A (Ed) Plant Tissue Culture 1982 (pp 399–400), Maruzen, Tokyo

    Google Scholar 

  • Gautheret RJ (1939) Sur la possibilité de réaliser la culture indéfinle des tissus de tubercules de carotte. C. R. Acad. Sci., Paris, 208:118–120

    Google Scholar 

  • Gautheret RJ (1941) Action du saccharose sur la croissance des tissus de carotte. C. R. Soc. Biol. 135:875–878

    Google Scholar 

  • Gupta R (1991) Agrotechnology of Medicinal Plants In: Wijseker ROB (Ed) The Medicinal Plant Industry (pp 43–57). CRC Press, Boca Raton, USA

    Google Scholar 

  • Haberlandt G (1902) Culturversuche mit isolierten Pflanzenzellen. Sitzungsberichte Math. Naturw. Kl. Kais. Akad. Wiss. Wien 111:69–92

    Google Scholar 

  • Hampp N & Zenk MM (1988) Homogeneous strictosidine synthase from cell suspension cultures ofRauwolfia serpentina. Phytochem. 27:3811–3815

    Google Scholar 

  • Hartmann T (1985) Principles of plant secondary metabolism. Plant Syst. Evol. 150:13–34

    Google Scholar 

  • Hashimoto T & Yamada Y (1992) Biosynthesis of scopolamine and its application for genetic engineering of medicinal plants. In: Oono R, Hirabayashi T, Kiruchi S, Handa H & Kahwara S (Eds) Plant Tissue Culture and Gene Manipulation for Breeding and Formation of Phytochemicals (pp 255–259) National Institute of Agrobiological Resources, Tsukuba, Japan

    Google Scholar 

  • Heide L & Tabata M (1987a) Enzyme Activities in Cell-Free Extracts of Shikonin-ProducingLithospermum erythrorhizon Cell Suspension Cultures. Phytochem. 26:1645–1650

    Google Scholar 

  • Heide L & Tabata M (1987b) Geranylpyrophosphate:p-Hydroxybenzoate Geranyltransferase Activity in Extracts ofLithospermum erythrorhizon Cell Cultures. Phytochem. 26:1651–1655

    Google Scholar 

  • Herbers K, Wilke I & Sonnewald U (1995) A thermostable xylanase fromClostridium thermocellum expressed at high levels in the apoplast of transgenic tobacco has no detrimental effects and is easily purified. Bio/Technology 13:63–66

    Google Scholar 

  • Inoue H, Ueda S, Inoue K & Matsumura H (1979) Biosynthesis of shikonin in callus cultures ofLithospermum erythrorhizon. Phytochemistry 18:1301–1308

    Google Scholar 

  • Kesselring (1985) Pflanzenzellkulturen (PZK) zur Auffindung neuer, therapeutisch relevanter Naturstoffe und deren Gewinnung durch Fermentationsprozesse. In: Bundesminister für Forschung und Technology (Ed) Pflanzliche Zellkulturen (pp 111–129). Projektträger Biotechnologie, KFA Jülich, Germany

    Google Scholar 

  • Kurz WGW, Tyler RT & Roewer IA (1990) Elicitation — A method to induce metabolite production by plant cells. In: Durand G, Bobichon L & Florent J (Eds) Proc. 8th Int. Biotechnol. Symp. vol. 1 (pp 193–204), Soc. Franc. Microbiol., Paris, France

    Google Scholar 

  • Kutchan TM (1989) Expression of enzymatically active cloned strictosidine synthase from the higher plantRauwolfia serpentina inEscherichia coli. FEBS Lett. 257:127–130

    Google Scholar 

  • Kutchan T, Dittrich H, Bracher D & Zenk MH (1991) Enzymology and molecular biology of alkaloid biosynthesis. Tetrahedron 47:5945–5954

    Google Scholar 

  • Lutterbach R & Stöckigt J (1992) High-yield formation of arbutin from hydroquinone by cell-susspension cultures ofRauwolfia serpentina. Helv. Chim. Acta 75:2009–2011

    Google Scholar 

  • Lutterbach R, Ruyter CM & Stöckigt J (1994) Isolation and characterization of an UDPG-dependent glucosyltransferase activity fromRauwolfia serpentina Benth. cell suspension cultures. Can. J. Chem. 72:51–55

    Google Scholar 

  • Mizukami H, Terato T, Miura H & Ohashi H (1983) Glucosylation of salicyl alcohol in cultured plant cells. Phytochem. 22:679–680

    Google Scholar 

  • Mizukami H, Terato T & Ohashi H (1985) Partial purification and characterization of UDP-glucose:salicyl alcohol glucosyltransferase fromGardenia jasminoides cell cultures. Planta Me. 46:104–107

    Google Scholar 

  • Mizukami H, Terato T, Amano A & Ohashi H (1986) Glucosylation of salicyl alcohol byGardenia jasminoides cell cultures. Plant Cell Physiol. 27:645–650

    Google Scholar 

  • Mizukami H, Terato T & Ohashi H (1987) Effect of substituent groups on the glucosyl formation of xenobiotic phenols by cultured cells ofGardenia jasminoides. Plant Sci. 48:11–15

    Google Scholar 

  • Nobécourt P (1937) Culture en série de tissus végétaux sur milieu artificiel. C.R. Séances Soc. Biol. 205:521–523

    Google Scholar 

  • Pabsch K, Petersen M, Rao NN, Alfermann AW & Wandrey C (1991) Chemo-enzymatic synthesis of rosmarinic acid. Rec. Trav. Chim. Pays-Bas 110:199–205

    Google Scholar 

  • Petersen M (1995) Plant cell cultures as a source of enzymes involved in natural product biosynthesis. Proc. 7th Europ. Congr. Biotechnol., Nice February 19–23, in press

  • Petersen M & Seitz HU (1985) Cytochrome P-450 dependent digitoxin 12β-hydroxylase from cell cultures ofDigitalis lanata. FEBS Lett. 188:11–14

    Google Scholar 

  • Petersen M & Seitz HU (1988) Reconstitution of cytochrome P-450 dependent digitoxin 12β-hydroxylase from cell cultures ofDigitalis lanata Ehrh. Biochem. J. 252:537–543

    Google Scholar 

  • Petersen M, Seitz HU & Reinhard E (1988) Characterisation and localisation ofdigitoxin 12β-hydroxylase from cell cultures ofDigitalis lanata Ehrh. Z. Naturforsch. 43c:199–206

    Google Scholar 

  • Petersen M, Alfermann AW, Reinhard E & Seitz HU (1987) Immobilization of a 12β-hydroxylase from cell suspension cultures ofDigitalis lanata Ehrh. Plant Cell Rep. 6:200–203

    Google Scholar 

  • Petersen M, Dombrowski K, Gertlowski C, Häusler E, Karwatzki B, Meinhard J & Alfermann AW (1992) The use of plant cell cultures to study natural product biosynthesis. In: Oono R, Hirabayashi T, Kiruchi S, Handa H & Kahwara S (Eds) Plant Tissue Culture and Gene Manipulation for Breeding and Formation of Phytochemicals (pp 297–310) National Institute of Agrobiological Resources, Tsukuba, Japan

    Google Scholar 

  • Pfitzner U & Zenk MH (1982) Immobilization of strictosidine synthase from Catharanthus cell cultures and preparative synthesis of strictosidine. Planta Med. 46:10–14

    Google Scholar 

  • Pilgrim H (1970) Untersuchungen zur Glycosidbildung in pflanzlichen Gewebekulturen. Pharmazie 25:568

    Google Scholar 

  • Reinert J, Clauss H & von Ardenne R (1964) Anthocyanbildung in Gewebekulturen vonHaplopappus gracilis in Licht verschiedener Qualität. Naturwiss. 51:87

    Google Scholar 

  • Romeike A (1960) Die Rolle von Sproβ und Wurzel bei der Umwandlung des Hyoscyamins in verschiedenenDatura Arten. Planta Medica 8:491–496

    Google Scholar 

  • Routien JB & Nickel LG (1952) Cultivation of Plant Tissue. US Patent 2,747,334

  • Ruyter CM & Stöckigt J (1989) Novel natural products from plant cell and tissue cultures—an update. GIT Fachz. Lab. 4:283–293

    Google Scholar 

  • Scott AI (1994) Genetically engineered synthesis of natural products. J. Nat. Prod. 57:557–573

    Google Scholar 

  • Stöckigt J (1993) Biotransformations with plant cells. Agro-Food-Industry Hi-Tech:25–28

  • Suzuki T, Yoshioka T, Tabata M & Fujita Y (1987) Potential ofDatura innoxia cell suspension cultures for glycosylating hydroquinone. Plant Cell Rep. 6:275–278

    Google Scholar 

  • Tabata M, Ikeda F, Hiraoka N & Konoshima M (1976) Glucosylation of phenolic compounds byDatura innoxia suspension cultures. Phytochem. 15:1225–1229

    Google Scholar 

  • Tabata M, Umetani Y, Ooya M & Tanaka S (1988) Glucosylation of phenolic compounds by plant cell cultures. Phytochem. 27:809–813

    Google Scholar 

  • Tanaka S, Hayakawa K, Umetani Y & Tabata M (1990) Glucosylation of isomeric hydroxybenzoic acids by cell suspension cultures ofMallotus japonicus. Phytochem. 29:1555–1558

    Google Scholar 

  • Treimer JF & Zenk MH (1979) Purification and properties of strictosidine synthase, the key enzyme in indole alkaloid formation. Eur. J. Biochem. 101:225–233

    Google Scholar 

  • Ushiyama K (1991) Large scale cultivation of ginseng. In: Komamine A, Misawa A & DiCosmo F (eds) Plant Cell Cultures in Japan (pp 92–98) CMC, Tokyo, Japan

    Google Scholar 

  • Yokoyama M & Yanagi M (1991) High level production of arbutin by biotransformation. In: Komamine A & Misawa M (Eds) Plant Cell Culture in Japan (pp 79–91). CMC, Tokyo, Japan

    Google Scholar 

  • Wahl J (1985) Adaption konventioneller Fermenter zur Züchtung von Pflanzenzellen zum Zwecke der Gewinnung von Naturstoffen. In: Bundesminister für Forschung und Technology (Ed.) Pflanzliche Zellkulturen (pp 35–43). Projektträger Biotechnologie, KFA Jülich, Germany

    Google Scholar 

  • Westphal K (1990) Large scale production of new biologically active compounds in plant cell cultures. In: Nijkamp HJJ, van der Plas LHW & van Aartrijk J (Eds) Progress in Plant Cellular and Molecular Biology (pp 601–608). Kluwer, Dordrecht, The Netherlands

    Google Scholar 

  • White PR (1939) Potentially unlimited growth of excised plant callus in an artificial medium. Am. J. Bot. 26:59–64

    Google Scholar 

  • Zenk MH, El-Shagi H, Arens H, Stöckigt J, Weiler EW & Deus B (1977) Formation of indole alkaloids serpentine and ajmalicine in cell suspension cultures ofCatharanthus roseus. In: Barz W, Reinhard E & Zenk MH (Eds) Plant Tissue Culture and its Bio-Technological Application (pp 27–43). Springer Verlag, Berlin, Germany

    Google Scholar 

  • Zenk MH (1991) Chasing the enzymes of secondary metabolism: Plant Cell Cultures as a pot of gold. Phytochem. 30: 3861–3863

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, D-40225, Düsseldorf, Germany

    A. W. Alfermann & Maike Petersen

Authors
  1. A. W. Alfermann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maike Petersen
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Dedicated to the memory of Prof. Dr. G. Haberlandt on the occasion of the 50th anniversary of his death

Rights and permissions

Reprints and permissions

About this article

Cite this article

Alfermann, A.W., Petersen, M. Natural product formation by plant cell biotechnology. Plant Cell Tiss Organ Cult 43, 199–205 (1995). https://doi.org/10.1007/BF00052176

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00052176

Keywords

Search

Navigation