Abstract
Two cell lines of Vinca major L. cv. variegata produced strictosidine lactam as the main alkaloid. Quantities varied from 500–1000 μg/g DW in the high-yielding line and 1–100 μg/g DW in the low-yielding line. Transfer of cells to alkaloid production medium resulted in a 6–8 fold increase in alkaloid production with the high-yielding line, some increase in the low-yielding line and in both lines induced intense greening (up to 200 μg chlorophyll/g DW) indicating chloroplast differentiation. Though in the source plant leaves are the main storage site, no correlation between alkaloid accumulation and chloroplast differentiation could be found.
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References
Balsevich J, Constabel F, Kurz WGW (1982) Alkaloids of Vinca major cv. variegata. Planta Med 44: 91–93
Zenk MH, El-Shagi H, Arens H, Stockigt J, Weiler EW Deus B (1977) Formation of the indole alkaloids serpentine and ajmalicine in cell suspension cultures of Catharanthus roseus. In: Barz W, Reinhard E, Zenk MH (eds) Plant Tissue Culture and its Bio-technological Application. Berlin, Heidelberg, New York: Springer Verlag, pp 27–43
Gamborg OL, Millar RH, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158
Metzner H, Rau H, Senger H (1965) Untersuchungen zur Synchronisierbareit einzelner Pigment mangel Mutanten von Chlorella. Planta 65: 186–194
Kurz WGW, Constabel F (1982) Production and isolation of secondary metabolites. In: Wetter LR, Constabel F (eds) Plant Tissue Culture Methods. NRC, pp 128–131
Farnsworth NR, Blomster RN, Damratoski D, Meer WA, Cammarato LV (1964) Studies on Catharanthus alkaloids. VI. Evaluation by means of thin-layer chromatography and ceric ammonium sulfate spray reagent. Lloydia, 27: 320–314
Kurz WGW (1984) Isolation and analysis of alkaloids. In: Vasil JK (ed) Cell Culture and somatic cell genetics of Plants. New York: Academic Press, Vol 1, pp 644–650
Kurz WGW, Constabel F, Kutney JP (1982) Biosynthesis and biotransformation of indole alkaloids in Catharanthus roseus cell cultures. In: Fujiwara H (ed) Plant Tissue Culture, Maruzen Co Ltd (pub), pp 361–364
Yamada Y, Sato F, Hagimori M (1978) Photoautotrophism in green cultured cells. In: Thorpe TA (ed) Frontiers of Plant Tissue Culture. IAPTC (pub), pp 452–462
Constabel F, Gaudet-La Prairie P, Kurz WGW, Kutney JP (1982) Alkaloid production in Catharanthus roseus cell cultures XII. Plant cell reports 1: 139–142
Barz W, Husemann W (1982) Aspects of photoautotrophic cell suspension cultures. In: Fujwara A (ed) Plant Tissue Culture. Maruzen Co Ltd (pub.), pp 245–248
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NRCC No. 26184.
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Eilert, U., Balsevich, J. & Kurz, W.G.W. Greening and strictosidine lactam production in two cell lines of Vinca major cv. variegata. Plant Cell Tiss Organ Cult 8, 177–182 (1987). https://doi.org/10.1007/BF00043154
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DOI: https://doi.org/10.1007/BF00043154