Summary
The single radial haemolysis (SRH) test was standardised for the detection of goat pox antigen and antibody. In this test soluble antigens, and serum prepared against soluble antigens were used for coupling sheep erythrocytes to detect goat pox antibody and antigen, respectively. This test was compared with the agar gel precipitation test (AGPT) and counter immunoelectrophoresis (CIE) test; the 2 most commonly used tests for goat pox diagnosis. SRH was found to be as sensitive as AGPT and CIE in detecting antigen and antibody (x2=2·9, P>0·05).
Résumé
Le test d'hémolyse radiale à simple diffusion a été standardisé pour la détection de l'antigéne et de l'anticorps de la variole caprine. Dans ce test, des antigénes solubles et un sérum préparés contre ces antigènes solubles ont été couplés à des hématies de mouton pur détecter respectivement l'anticorps et l'antigène de la variole caprine. Ce test a été comparé avec le test de précipitation en gélose (AGPT) et celui de contre-immunoélectrophorese (CIE), les deux tests les plus communément utilisés pour le diagnostic de la variole caprine. Le test d'hémolyse radiale s'est montré etre aussi sensible que l'AGPT et le CIE pour mettre en évidence l'anticorps et l'antigène (X2=2,9;P>0,05).
Resumen
El test de hemólisis radial simple (HRS) fue estandarizado para detectar antígenos y anticuerpos de la viruela caprina. Se utilizaron antígenos solubles y suero frente a antigenos solubles para conjugar eritrocitos de oveja con objeto de detectar respectivamente anticuerpos y antígenos de viruela caprina. El mencionado test se comparó con el de precipitación en agar gel (TPAG) y con el de contrainmunoelectroforesis (CIE), que son los dos tests más comunmente utilizados para diagnosticar viruela caprina. El test HRS resultó ser tan sensible como el test de CIE y el TPAG a la hora de detectar antigenos y anticuerpos (X2=2·9,P>0·05).
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Tiwari, A.K., Negi, B.S. Single radial haemolysis for the detection of goat pox virus antigen and antibody. Trop Anim Health Prod 28, 117–120 (1996). https://doi.org/10.1007/BF02250735
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DOI: https://doi.org/10.1007/BF02250735