Abstract
Addition of anti-immunoglobulin M (anti-IgM), G (anti-IgG) and A (anti-IgA) sera to the haemagglutination-inhibition (HI) test (anti-Ig HI test) forMycoplasma gallisepticum resulted in 2- to 8-fold increases in the HI titres. On investigating the anti-Ig HI reaction using IgM and IgG antibodies separated by affinity chromatography, it was confirmed that, in the enhanced HI titres, specificity existed between the chicken Ig classes having antibody activity and the antisera used in the test. Four days after inoculation ofM. gallisepticum, the anti-Ig HI reaction was markedly enhanced by anti-IgM antiserum in the intravenously inoculated chickens and by anti-IgA serum in the nasally inoculated chickens. Ten days after inoculation ofM. gallisepticum marked enhancement of the reaction was produced by anti-IgG serum in both intravenously and nasally inoculated chickens, but the enhancement of the anti-Ig HI reaction diminished from the second week after inoculation.
Similar content being viewed by others
References
Adler, H.E. and DaMassa, A.J., 1964. Enhancement ofMycoplasma agglutination titers by use of anti-globulin.Proceedings of the Society for Experimental Biology and Medicine,116, 608–610
Ashe, W.K. and Notkins, A.L., 1966. Neutralization of an infectious herpes simplex virus-antibody complex by anti-γ-globulin.Proceedings of the National Academy of Sciences of the USA,26, 447–451
Avrameas, S. and Ternynck, T., 1969. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.Immunochemistry,6, 53–66
Barbour, E.K., Newman, J.A., Sivanandan, V., Halvorsen, D.A. and Sasipreeyajan, J., 1987. Protection and immunity in commercial chicken layers administeredMycoplasma gallisepticum liposomal bacterins.Avian Diseases,31, 723–729
Baumer, J., 1975. Anti-immunoglobulin G in the haemagglutination inhibition test for Newcastle disease virus antibody assays.Avian Pathology,4, 45–51
Bradley, L.D., Synder, D.B. and Van Deusen, R.A., 1988. Identification of species-specific and interspecies-specific polypeptides ofMycoplasma gallisepticum andMycoplasma synoviae.American Journal of Veterinary Research,49, 511–515
Coombs, R.R.A. and Stocker, M.G.P., 1951. Detection of Q-fever antibodies by the anti-globulin sensitisation test.Lancet,2, 15–17
Coombs, R.R.A., Mourant, A.E. and Race, R.R., 1945. A new test for the detection of weak and ‘incomplete’ RH agglutinins.British Journal of Experimental Pathology,26, 255–266
Doria, G., D'agostaro, G. and Poretti, A., 1978. Age-dependent variations of antibody avidity.Immunology,35, 601–611
Hahon, N., 1970. Neutralization anti-IgG test for antisera to Venezuelan equine encephalomyelitis.Journal of General Virology,6, 285–291
Hahon, N., Booth, J.A. and Eckert, H.L., 1971. Anti-immunoglobulin G hemagglutination-inhibition test for influenza.Infection and Immunity,4, 508–510
Hahon, H., Booth, J.A. and Eckert, H.L., 1972. Further studies on the anti-immunoglobulin G hemagglutination-inhibition test for influenza.Applied Microbiology,23, 485–489
Kuniyasu, C. and Ando, K., 1966. Studies on the hemagglutination-inhibition test forMycoplasma gallisepticum infection of chickens.National Institute of Animal Health Quarterly,6, 136–143
Kuniyasu, C. and Yoshida, Y., 1972. Cold hemagglutination in serum of chicken infected withMycoplasma gallisepticum.National Institute of Animal Health Quarterly,12, 119–128
Kuniyasu, C., Matsui, K., Ando, K. and Yoshida, T., 1967. Serological responses of chickens naturally infected withMycoplasma gallisepticum and the effect of tylosin on these responses.National Institute of Animal Health Quarterly,7, 57–64
Leslie, G.A. and Benedict, A.A., 1968. Nonhemagglutinating nondissociable 7S subunits of chicken IgM antibody.Proceedings of the Society for Experimental Biology and Medicine,128, 1012–1016
Leslie, G.A. and Clem, L.W., 1969. Phylogeny of immunoglobulin structure and function. III. Immunoglobulins of the chicken.Journal of Experimental Medicine,130, 1337–1352
Mancini, G., Carbonara, A.O. and Heremans, J.F., 1965. Immunochemical quantitation of antigens by single radial immunodiffusion.Immunochemistry,2, 235–254
Moreschi, C., 1908. Neue Tatsachen über die Blutkörpercheagglutination.Zentralblatt für Bakteriologie, Parasitenkunde und Infektionskrankheiten. Abteilung 1,46, 49–51
Notkins, A.L., Ashe, W.K. and Mahar, S., 1968. Neutralization of sensitized lactic hydrogenase virus by anti-γ-globulin.Journal of Immunology,100, 314–320
Radwan, A.I., Burger, D. and Davis, W.C., 1973. The fate of sensitized equine arthritis virus following neutralization by complement or anti-IgG serum.Virology,53, 372–378
Stewart, F.S. and McKeever, J.D., 1950. The antiglobulin technique applied to the detection of non-agglutinating antibody againstSalmonella typhi O in human sera.Journal of Hygiene,48, 357–360
van Eijk, H.G. and van Noort, W.L., 1976. Isolation of rat transferrin using CNBr-activated Sepharose 4B.Journal of Clinical Chemistry and Clinical Biochemistry,14, 475–478
Watanabe, H. and Kobayashi, K., 1974. Peculiar secretory IgA system identified in chickens.Journal of Immunology,113, 1405–1409
Yagihashi, Y. and Tajima, M., 1986. Antibody responses in sera and respiratory secretions from chickens infected withMycoplasma gallisepticum.Avian Diseases,30, 543–550
Yamamoto, S., 1977. Studies on enhancement of the sensitivity of hemagglutination-inhibition test forMycoplasma gallisepticum by anti-chicken immunoglobulin serum, (Thesis, Azabu Veterinary College, Kanagawa, Japan)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamamoto, S. The effect of including anti-Ig sera in the haemagglutination inhibition test forMycoplasma gallisepticum . Vet Res Commun 16, 185–193 (1992). https://doi.org/10.1007/BF01839154
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01839154