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Purification and properties of β-amylase from Bacillus circulans S31

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Abstract

A thermotolerant β-amylase was purified from Bacillus circulans S31 isolated from soil in Hong Kong. The purified enzyme has an M r of 64 kDa and was stable at 50°C and pH 7.0 for 30 min. Its K m for starch was 0.9 mg/ml with a V max of 0.3 mg/min. It was not activated by any metal ion although sulphydrys reagents were inhibitory.

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Authors
  1. H. S. Kwan
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  2. K. H. So
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  3. K. Y. Chan
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  4. S. C. Cheng
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Additional information

H.S. Kwan, K.H. So and K.Y. Chan are with the Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong S.C. Cheng is with the Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic, Hung Hom, Hong Kong.

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Kwan, H.S., So, K.H., Chan, K.Y. et al. Purification and properties of β-amylase from Bacillus circulans S31. World J Microbiol Biotechnol 10, 597–598 (1994). https://doi.org/10.1007/BF00367677

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  • DOI: https://doi.org/10.1007/BF00367677

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