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Glucose oxidase biosynthesis using immobilized mycelium of Aspergillus niger

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Abstract

A simple method is described for the immobilization of Aspergillus niger GIV-10 which produces an extracellular glucose oxidase. A. niger conidia were immobilized on sintered glass Raschig rings, pumice stones or polyurethane foam. Mycella growing out from the spores produced extracellular glucose oxidase: the highest production was with the pumice stone carrlers. This technique facilitates the growth of the filamentous cultures in the spongy structure of a support with continuous accumulation of biomass. After 24 to 36 h, a culture liquid with 2.7 to 3.1 U of glucose oxidase/ml was obtained. This procedure also made possible repeated batch enzyme production and as many as 25 subsequent 24-h batches could be fermented by using the same carrier with only a small loss of glucose oxidase activity.

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References

  • Bajpai, P. & Margaritis, A. 1987 The effect of temperature and pH on ethanol production by free and immobilized cells of Kluyveromyces marxianus grown on Jerusalem artichoke extract. Biotechnology and Bioengineering 30, 306–313.

    Google Scholar 

  • Bisping, B. & Rehm, H.J. 1986 Glycerol production by cells of Saccharomyces cerevisiae immobilized in sintered glass. Applied Microbiology and Biotechnology 23, 174–179.

    Google Scholar 

  • Bliyeva, R.K. & Bielkhodyaieva, A.A. 1989 Immobilization of Aspergillus awamori producing pectin-decomposing enzymes. Abstracts of International Conference ‘Biotechnology and Food”, sponsored by IUFoST, 20–24 February, Hohenheim University, Stuttgart.

    Google Scholar 

  • Dawidowicz, A. 1988 Sposób otrzymywania materiaŀów porowatych. Polish Patent No. P272278.

  • Fiedurek, J. & Łobarzewski, J. 1990 Glucomylase biosynthesis by cells of Aspergillus niger immobilized in sintered glass and pumice stones. Starch/Stärke 42, 358–362.

    Google Scholar 

  • Fiedurek, J., Rogalski, J. & Ilczuk, Z. 1990 Intensification of glucose oxidase synthesis with Aspergillus niger by way of multistage mutagenization. Acta Biotechnologica 10, 357–362.

    Google Scholar 

  • Fiedurek, J., Rogalski, J., Ilczuk, Z. & Leonowicz, A. 1986 Screening and mutagenesis of moulds for the improvement of glucose oxidase production. Enzyme and Microbial Technology 8, 734–736.

    Google Scholar 

  • Kokubu, T., Karube, J. & Suzuki, S. 1978 α-Amylase production by immobilized whole cells of Bacillus subtilis. European Journal of Applied Microbiology and Biotechnology 5, 233–240.

    Google Scholar 

  • Kokubu, T., Karube, J. & Suzuki, S. 1981 Protease production by immobilized mycelia of Streptomyces fradiae. Biotechnology and Bioengineering 23, 29–39.

    Google Scholar 

  • Linko, P. & Linko, Y.Y. 1984 Industrial application of immobilized cells. Critical Review in Biotechnology 1, 289–338.

    Google Scholar 

  • Nelson, N.J. 1944 A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153, 375–380.

    Google Scholar 

  • Rogalski, J., Fiedurek, J., Czczodrak, J., Kapusta, K. & Leonowicz, A. 1988 Optimization of glucose oxidase synthesis in submerged cultures of Aspergillus niger G-13 mutant. Enzyme and Microbial Technology 10, 508–511.

    Google Scholar 

  • Schacterle, G.R. & Pollack, R.L. 1973 Simplified method for the quantitative assay of amounts of protein in biologic material. Analytical Biochemistry 51, 654–655.

    Google Scholar 

  • Tamada, M., Kasai, N. & Kaetsu, I. 1987 Continuous cellulase production by immobilized Sporotrichum cellulophilum and continuous saccharification of bagasse. Biotechnology and Bioengineering 30, 697–702.

    Google Scholar 

  • Tamada, M., Kasai, N. & Kaetsu, I. 1989 Improvement of cellulase activity by immobilization of Sporotrichum cellulophilum. Biotechnology and Bioengineering 33, 1343–1346.

    Google Scholar 

  • Tsay, S.S. & To, K.Y. 1987 Citric acid production using immobilized conidia of Aspergillus niger TMB 2022. Biotechnology and Bioengineering 29, 297–304.

    Google Scholar 

  • Younes, G., Nicaud, I.M. & Guespin-Michel, I. 1984 Enhancement of extracellular enzymic activities produced by immobilized growing cells of Myxococcus xanthus. Applied Microbiology and Biotechnology 19, 67–69.

    Google Scholar 

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Authors
  1. J. Fiedurek
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  2. Z. Ilczuk
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Additional information

The authors are with the Institute of Microbiology, M. Curie-Sklodowska University, Akademicka 19, 20-003 Lublin, Poland.

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Fiedurek, J., Ilczuk, Z. Glucose oxidase biosynthesis using immobilized mycelium of Aspergillus niger . World Journal of Microbiology and Biotechnology 7, 379–384 (1991). https://doi.org/10.1007/BF00329406

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  • DOI: https://doi.org/10.1007/BF00329406

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