Summary
The kinetics of enzymatic transformation of total glycoalkaloids (TGA) in haulm and tuber sprouts of potato to solanidine and the kinetics of solanidine isolation from plant material were studied. The optimum reaction conditions for the enzymatic TGA transformation were found for haulm to be: plant material to liquid phase 1:4 w/v, temperature 35°C, pH 5.4; and for tuber sprouts: plant material to liquid phase 1:3 w/v, temperature 37°C, pH 5.4. The. maximum degree of enzymatic transformation of TGA in haulm and tuber sprouts of 65 and 75%, respectively, was achieved after 50h. Carbon tetrachloride was the best solvent for solanidine extraction from the plant materials. The optimum ratio of plant material to carbon tetrachloride for the isolation of solanidine was 1:8 w/v for both extraction cycles, ensuring a yield of solanidine of approximately 55 and 65% from haulm and tuber sprouts, respectively, after 30 min.
Similar content being viewed by others
References
Bushway, R.J., E.S. Barden, A.W. Bushway & A.A. Bushway, 1980. The mass extraction of potato glycoalkaloids from blossoms.American Potato Journal 57: 175–180.
Coxon, D.T., R.K. Price & P.G. Jones, 1979. A simplified method for determination of total glycoalkaloids in potato tubers.Journal of the Science of Food and Agriculture 32: 1043–1049.
Fieser, L. & M. Fieser, 1967. Steroids. Reinhold Publishing Corporation. New York. Amsterdam, London.
Filadelfi, M.A. & A. Zitnak, 1982. Preparation of chaconines by enzymic hydrolysis of potato berry alkaloids.Phytochemistry 21: 250–251.
Filadelfi, M.A. & A. Zitnak, 1983. A simple TLC standard for identification of potato glycoalkaloids.Canadian Institute of Food Science and Technology Journal 16: 151–153.
Gelder, W.M.J. van, 1984. A new hydrolysis technique for steroid glycoalkaloids with unstable aglycone fromSolanum spp.Journal of Science of Food and Agriculture 35: 487–494.
Gregory, P., S.L. Linden, S.F. Osman, W.N. Tingey & D.A. Chessin, 1981. Glycoalkaloids of wild tuber-bearingSolanum species.Journal of Agriculture and Food Chemistry 29: 1212–1214.
Guseva, A.R. & V.A. Paseshnichenko, 1957. Enzymatic degradation of potato glycoalkaloids.Biochemistry 22: 792–799.
Petrochenko, E.I., 1953. Solaninase in potato sprouts.Doklady Akademii Nauk SSSR 90: 1091–1093.
Pogorelova, O.V., 1968. Farmakognosticheskoe isledovanie rostkov kartofelyaSolanum tubersosum L. kak istochnikov solanina.Farmatsia 4: 27–33.
Prelog, V. & O. Jeger, 1960. Steroid alkaloids: theSolanum group. In: F.H.R. Manske, The Alkaloids, Vol. 7. Academic Press, New York, London.
Roth, H.J. & L. Hornhammer, 1979. Hagers Handbuch der pharmazeutischen Praxis, vollständige (vierte) Neuausgabe, Band VI. Springer Verlag, Berlin, Heidelberg, New York.
Stanković, M., O. Stojanović & N. Kobilarov, 1990. Unsaponifiable lipids from haulm and tuber sprouts of potato (Solanum tuberosum L.).Potato Research 33: 459–464.
Swain, A.P., T.J. Fitzpatrick, E.A. Talley, S.F. Herb & S.F. Osman, 1978. Enzymatic hydrolysis of α-solanine and α-chaconine.Phytochemistry 17: 800–801.
Tukalo, E.A. & G.N. Tsarik, 1970. Uskorenii metod kolichestvenogo opredelenia glikoalkaloidov kartofelya.Biologicheskie Nauki 12: 115–118.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stanković, M.Z., Nikolić, N.C., Palić, R. et al. Isolation of solanidine from haulm and tuber sprouts of potato (Solanum tuberosum L.). Potato Res 37, 271–278 (1994). https://doi.org/10.1007/BF02360519
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02360519