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Molecular dynamics of agarose gels investigated by NMR and rayleigh scattering of Mössbauer radiation

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Il Nuovo Cimento D

Summary

NMR, X-ray and Rayleigh scattering of Mössbauer radiation (RSMR) measurements have been performed on agarose-water systems for different levels of water content. The NMR data provided information on the relaxation rates of protons in both polysaccharide and solvent. The RSMR measurement allowed us to determine the mean square atomic displacements <u 2> as a function of the scattering vector. The <u 2> values of carbon and oxygen atoms are larger when measured at distances corresponding to the helix diameter than along the chain bonds. At low hydration levels, the water molecules closely associated to the polysaccharide chains follow the dynamics of the polymer. A marked change in the dynamics of both polymer and water has been evidenced when the water weight exceeds 20% of the total weight of the sample.

Riassunto

Sono state eseguite misure NMR, di diffrazione di raggi X e di scattering Rayleigh della radiazione Mössbauer (RSMR) su sistemi agarosio-acqua con differenti gradi di idratazione. I dati NMR hanno fornito informazioni sulla velocità di rilassamento dei protoni, sia nel polisaccaride che nel solvente. Le misure RSMR hanno consentito di determinare lo spostamento atomico quadratico medio <u 2> in funzione del vettore di scatteringQ. I valori di <u 2> per gli atomi di carbonio e di ossigeno misurati per valori diQ corrispondenti alle distanze caratteristiche del diametro delle eliche sono maggiori di quelli relativi alle distanze tipiche dei legami intracatena. Per bassi valori di idratazione le molecole di acqua legate direttamente alle catene polisaccaridiche seguono la dinamica del polimero. Una marcata variazione nella dinamica, sia del polimero che dell'acqua, è stata osservata in campioni in cui l'acqua presente superava il 20% del peso totale del campione.

Резюме

Проводятся измерения ЯМР, рентгеновской дифракции и рэлеевского рассеяния Мёссбауэровского излучения на системах агара с водой для различных уровней содержания воды. Данные ЯМР обеспечивают информацию о скоростях релаксации протонов в полисахариде и в растворителе. Измерения рэлеевского рассеяния Мёссбауэровского излучения позволяют определить средние квадратичные смещения атомов (<u 2>), как функцию вектора рассеяния. Значения <u 2> для атомов углерода и кислорода, измеренные на расстояниях, соответствующих диаметру спирали, оказываются больше значений смещений, измеренных вдоль связей цепочек. При низких уровнях гидратации молекулы, воды, тесно связанные с цепочками полисахарида, следуют динамике поимера. Заметное изменение динамики полимера и воды обнаруживается, когда содержание воды превышает 20% от полного веса образца.

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Authors and Affiliations

  1. Dipartimento di Fisica, Università di Parma, Parma, Italia

    G. Albanese & A. Deriu

  2. Istituto di Strutturistica Chimica e Centro di Strutturistica Diffrattometrica del C.N.R., Università di Parma, Parma, Italia

    F. Ugozzoli

  3. Centro Interfacoltà di Misure, Università di Parma, Parma, Italia

    C. Vignali

Authors
  1. G. Albanese
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  2. A. Deriu
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  3. F. Ugozzoli
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  4. C. Vignali
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Albanese, G., Deriu, A., Ugozzoli, F. et al. Molecular dynamics of agarose gels investigated by NMR and rayleigh scattering of Mössbauer radiation. Il Nuovo Cimento D 9, 319–335 (1987). https://doi.org/10.1007/BF02458064

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  • DOI: https://doi.org/10.1007/BF02458064

PACS. 87.15He

PACS. 76.80

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