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Activation of interleukin-2 gene transcription by nuclear factors from the spleen and brain of immunized rats

  • Experimental Genetics
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Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The nuclear protein factors which stimulate synthesis of mRNA of interleukin-2 (IL-2) in the system of surviving murine T lymphocytes are isolated from cells of the spleen and brain of immunized rats. The active fractions of spleen and brain (13–19 and 25 kD, respectively), which interact with the promoter-enhancer sequences of the IL-2 gene localized in the region from +39 to −141 base pairs, are isolated by the method of high performance liquid chromatography from the total nuclear extract. The induction of luciferase synthesis is shown for protein factor-treated Jurkat cells transformed with p-IL-2LUC recombinant plasmid.

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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, No 5, pp. 523–526, May, 1994

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Kazakova, T.B., Grishina, T.V., Golovko, O.I. et al. Activation of interleukin-2 gene transcription by nuclear factors from the spleen and brain of immunized rats. Bull Exp Biol Med 117, 522–525 (1994). https://doi.org/10.1007/BF02444307

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  • DOI: https://doi.org/10.1007/BF02444307

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