Abstract
A method of cultivating and fertilizing human ova obtained from ovaries removed at operation is described. A special method of sampling active spermatozoa was used for fertilization. Zygote formation was observed 24–36 h after fertilization of the ripe ova, an embryo of the two-cell stage was formed after 36–40 h, and an embryo at the four-cell stage after 48 h. The number of blastomeres reached 8 after 60–64 h, and after approximately 100–120 h the embryos reached the morula stage. The results provide wide scope for the study of the physiology and pathology of early human embryonic development.
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Nikitin, A.I., Nikolaichuk, M.P. & Kitaev, É.M. Fertilization and cleavage of the human ovumin vitro . Bull Exp Biol Med 81, 451–453 (1976). https://doi.org/10.1007/BF00804947
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DOI: https://doi.org/10.1007/BF00804947