Abstract
In vitro DNA:DNA hybridizations and hydroxyapatite thermal-elution chromatography were employed to identify the diploid Triticum species ancestral to the G genome of Triticum timopheevii. Total genomic, unique-sequence, and repeated-sequence fractions of 3H-T. timopheevii DNA were hybridized to the corresponding fractions of unlabeled DNAs of T. searsii, T. speltoides, T. sharonensis, T. longissimum, and T. bicorne. The heteroduplex thermal stabilities indicated that, of the five species examined, T. speltoides was the most closely related to the G genome of T. timopheevii. Thus, T. spelotides appears to be the G-genome donor to T. timopheevii. The thermal stability profiles further indicated that the repeated DNA fractions from the five diploid species and the tetraploid T. timopheevii are more similar than the unique DNA fractions. This indicates that all of these species are closely related and that the sequences which comprise the current repeated fractions in the various species have not undergone any significant change since the formation of various species.
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References
Bendich, A. J., and McCarthy, B. J. (1970a). DNA comparisons among barley, oats, rye and wheat. Genetics 65545.
Bendich, A. J., and McCarthy, B. J. (1970b). DNA comparisons among some biotypes of wheat. Genetics 65567.
Bernardi, G. (1965). Chromatography of nucleic acids on hydroxyapatite. Nature 206779.
Britten, R. J., and Kohne, D. E. (1968). Repeated sequences in DNA. Science 161529.
Britten, R. J., and Smith, J. (1970). A bovine genome. Carnegie Inst. Wash. Yearbk. 68378.
Feldman, M. (1966). The effect of chromsomes 5B, 5D and 5A on chromosomal pairing in Triticum aestivum. Proc. Natl. Acad. Sci. 551447.
Flavell, R. (1980). The molecular characterization and organization of plant chromosomal DNA sequences. Annu. Rev. Plant Physiol. 31569.
Gill, B. S., and Kimber, G. (1974). Giemsa C-banding and the evolution of wheat. Proc. Natl. Acad. Sci. 71486.
Kihara, H. (1924). Cytologische and genetische Studien bei wichtigen Getreidearten mit besondere Rucksicht auf das Verhalten der Chromosomen in den Bastarden. Memorial College of Science, Kyoto Imperial University, Service Bulletin 1, p. 200.
Kihara, H. (1944). Die Entdeckung der DD: Analysatoren beim Weizen. Agr. Hort. (Tokyo) 19889.
Kimber, G. (1974). A reassessment of the origin of the polyploid wheats. Genetics (Suppl. 78487.
Kohne, D. E. (1969). Isolation and characterization of bacterial ribosomal RNA cistrons. Carnegie Inst. Wash. Yearbk. 67310.
Kohne, D. E., and Britten, R. J. (1971). Hydroxyapatite techniques for nucleic acid reassociation. In Cantoni, G. L., and Davies, D. R. (eds.), Procedures in Nucleic Acid Research Harper and Rowe, New York, p. 500.
Laird, C. D., McConaughy, B. L., and McCarthy, B. J. (1969). On the rate of fixation of nucleotide substitutions in evolution. Nature 244149.
Lilienfeld, F., and Kihara, H. (1934). Genomanalyse bei Triticum und Aegilops V. Triticum timopheevii Zhuk. Cytologia 687.
Love, R. M. (1941). Chromosome behavior in F1 wheat hybrids I. Pentaploids. Can. J. Res. 19351.
Maan, S. S., and Lucken, K. A. (1972). Interacting male-sterility male-fertility restoration systems for hybrid wheat research. Crop Sci. 1230.
Marmur, J. (1961). A procedure for the isolation of DNA from microorganisms. J. Mol. Biol. 3208.
McFadden, E. S., and Sears, E. R. (1946). The origin of Triticum spelta and its free-threshing hexaploid relatives. J. Hered. 3781.
Nath, J., McNay, J. W., Paroda, C., and Gulati, S. (1983). Implication of Triticum searsii as the B-genome donor to wheat using DNA hybridizations. Biochem. Genet. 21745.
Nath, J., Hanzel, J., Thompson, J. P., and McNay, J. W. (1984). Additional evidence implicating Triticum searsii as the B-genome donor to wheat. Biochem. Genet. 2237.
Paroda, C. (1976). Studies on B Genome in Polyploid Wheat by Nucleic Acid Hybridizations Ph. D. thesis, West Virginia University, Morgantown.
Sachs, L. (1953). Chromosome behavior in species hybridized with T. timopheevii. Heredity 749.
Sax, K. (1922). Sterility in wheat hybrids. II: Chromosome behavior in partially sterile hybrids. Genetics 7513.
Sears, E. (1948). Cytology and genetics of the wheats and their relatives. Adv. Genet. 2239.
Shands, H., and Kimber, G. (1973). Reallocation of the genomes of Triticum timopheevii Zhuk. International Wheat Genetics Symposium, Columbia, Mo., p. 101.
Wagenaar, E. B. (1961). Studies on the genome constitution of Triticum timopheevii Zhuk. I. Evidence for genetic control of meiotic irregularities in tetraploid hybrids. Can. J. Genet. Cytol. 347.
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Published with the approval of the Director of the West Virginia Agriculture and Forestry Experiment Station as Scientific Paper No. 1850.
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Nath, J., Thompson, J.P. & Gulati, S.C. Identification of the G-genome donor to Triticum timopheevii by DNA:DNA hybridizations. Biochem Genet 23, 125–137 (1985). https://doi.org/10.1007/BF00499118
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DOI: https://doi.org/10.1007/BF00499118