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Detection of microsatellite alterations in nipple discharge accompanied by breast cancer

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Abstract

Nipple discharge in breast cancer cases was examined loss of heterozygosity (LOH). DNA samples were extracted from both supernatant and cell pellet components of the discharge, and examined for LOH at microsatellite markers, D11S1818, D11S2000, D16S402, D16S504, D16S518, D17S520, and D17S786. At least one LOH was found in either the supernatant or cell pellet in seven out of 10 patients (70%). Five of seven samples, which were cytologically negative, were LOH positive, and only one case, which was cytologically positive, showed no LOH on the markers examined. All three samples, which were judged ‘negative’ by CEA measurement (<400 ng/ml), were LOH positive. This method could be a useful novel diagnostic modality for nonpalpable breast cancer with nipple discharge.

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Authors and Affiliations

  1. Department of Surgery II, Osaka University Medical School, Yamadaoka, Suita, Osaka, Japan

    Michihiko Miyazaki, Yasuhiro Tamaki, Isao Sakita, Yoshiyuki Fujiwara, Masao Kadota, Norikazu Masuda, Masaru Ooka, Tadashi Ohnishi, Masayuki Ohue, Mitsugu Sekimoto, Naohiroi Tomita & Morito Monden

  2. Department of Surgery, Municipal Suita Hospital, Katayamacho, Osaka, Japan

    Junkou Furukawa

  3. Department of Pathology, School of Allied Health Sciences, Osaka University Medical School, Yamadaoka, Suita, Osaka, Japan

    Nariaki Matsuura

Authors
  1. Michihiko Miyazaki
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  2. Yasuhiro Tamaki
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  3. Isao Sakita
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  4. Yoshiyuki Fujiwara
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  5. Masao Kadota
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  6. Norikazu Masuda
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  9. Masayuki Ohue
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  11. Naohiroi Tomita
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  13. Nariaki Matsuura
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  14. Morito Monden
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Miyazaki, M., Tamaki, Y., Sakita, I. et al. Detection of microsatellite alterations in nipple discharge accompanied by breast cancer. Breast Cancer Res Treat 60, 35–41 (2000). https://doi.org/10.1023/A:1006336110322

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