Abstract
The diagnostic value of the decrease in percentage of immunoglobulin G1 (%IgG1) in breast cancer was analyzed with special emphasis on early tumor stages. IgG1 and total IgG were preoperatively measured in the sera of a total of 801 individuals using a modified quantitative affinity chromatography. Group A consisted of 174 healthy individuals of both sexes, group B of 324 female patients with benign breast disease, and group C of 303 patients with invasive and non-invasive breast cancer. Within group C, 13 patients presented with intraductal carcinoma, and 22 patients with a pT1a-tumour (diameter less than 0.5 cm). The %IgG1 values were compared among groups A, B and C. In addition, correlations were sought between %IgG1 values of group C and tumor size, stage (UICC), histopathological grade and oestrogen (ER) and progesteron receptor (PR) expression. The mean value of %IgG1 in group A was 63.3 ± 0.5 s.e.m., in group B 57.75 ± 0.4 s.e.m. and in group C 52.37 ± 0.5 s.e.m. The differences of mean values were highly significant between all three groups. Sensitivity and specificity of %IgG1 to discriminate between group A and C were 75% and 87%, and between group B and C 62% and 63%, respectively. The significant decrease of %IgG1 in total serum IgG is able to distinguish patients with breast cancer of more than 5 mm in diameter from healthy controls and patients with benign breast diseases. Finally, calculated posterior probabilities revealed that within certain concentration limits %IgG1 may provide predictive information with high xprobabilities.
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Sölétormos G, Nielsen D, Schioler V, Skovsgaard T, Winkel P, Mouriden HT, Dobernowsky P: A novel method for monitoring high-risk breast cancer with tumor markers: CA 15.3 compared to CEA and TPA. Ann Oncol 4: 861–869, 1993
Schauenstein E, Dachs F, Reiter M, Gombotz H, List W: Labile disulfide bonds and free thiol groups in human IgG-I. Assignment to IgG1 and IgG2 subclasses. Int Arch Allergy Appl Immunol 80: 174–179, 1986
Schauenstein E, Reiter M, Gombotz H, List W: Labile disulfide bonds and free thiol groups in human IgG. II. Characteristic changes in malignant diseases corresponding to shifts of IgG1 and IgG2 subclasses. Int Arch Allergy Appl Immuno 80: 180–184, 1986
Schauenstein E, Lahousen M, Weblacher M, Steinschifter W, Estelberger W, Schauenstein K: Selective decrease in serum immunoglobulin G1. A tissue nonspecific tumor marker detecting early stages of gynecologic malignant disease with high efficiency. Cancer 78: 511–516, 1996
Kronberger L Jr, Weblacher M, Estelberger W, Hauser H, Schauenstein E, Schauenstein K, Smola M, Steindorfer P: Preand postoperative analysis of serum IgG1 in patients with benign and malignant breast disease. In Kubista E, Staffen A, Zielinski C (eds), Int Proc Div, 2nd Europ Conress on Senology. Monduzzi Editore 1994, pp 479–485
Smola M, Estelberger W, Reiter M, Schauenstein K, Schauenstein E: ΣS, a measure of reactive sulfur groups in immunoglobulin G, is a sensitive tumor marker discriminating different stages of breast cancer. Cancer 68: 1026–1030, 1991
Anderhuber W, Steinschifter W, Schauenstein E, Gotschuli A, Habermann W, Fischer M, Felsner P, Schauenstein K: The IgG1/G2 subclass shift - a sensitive, tissue nonspecific marker for malignancy. Diagnostic performance with squamous cell carcinoma of the head and neck. Br J Cancer 79: 1777–1781, 1999
Schauenstein E, Rabl H, Steinschifter W, Hirschmann C, Estelberger W, Schauenstein K: Selective decrease of serum immunoglobulin G1 as a marker of malignant transformation in colorectal tissue. Cancer 79: 1482–1486, 1997
Tavassoli FA: Pathology of the Breast, 2nd edn, Appelton & Lange, Stamford, CT 1999, pp 402
World Health Organisation: Histologic Typing of Breast Tumors, International Histologic Classification of Tumors, 2nd edition Geneva, 1981
UICC TNM: Classification of Malignant Tumors, 4th edition, In: Sobin, LH and Wittekind CH (eds). Wiley, New York, 1997
ACO/ASSO: Arbeitsgemeinschaft für Chirurgische Onkologie der Österreichischen Gesellschaft für Chirurgie, Consensus Bericht Mammacarcinom, Smola MG (ed.), 1993
Remmele W, Schicketanz KH: Immunohistochemical determination of estrogen and progesterone receptor content in human breast cancer. Computer-assisted image analysis (QIC score) vs subjective grading (IRS). Pathol Res Pract 189: 862–866, 1993
Maninger K, Schauenstein K, Weblacher M, Tillian H, Schauenstein E: Shift in IgG subclasses - a sensitive early marker for malignant proliferation in tumor bearing rats as a representive model for human cancer. Cancer Mol Biol 1: 165–171, 1994
French M.H, Harrison G: Serum IgG subclass concentrations in healthy adults: a study using monoclonal antisera. Clin Exp Immunol 56: 473–475, 1984
Weblacher M, Leitsberger A, Tillian H, Maninger K, Estelberger W, Schauenstein K, Schauenstein E: A decrease in reactive disulphide bonds of serum IgG signals a characteristic change in the IgG subclass pattern of rats bearing experimental tumors. Int Arch Allergy Appl Immunol 102: 340–346, 1993
Majus J, Kaine J, Nahm G: IgG subclass levels and disease activity in systemic lupus. Fed Proc 44: 1716, 1985
Stanworth DR: IgG subclasses in rheumatoid joint disorders. Monographs Allergy 19, Kager Basel 188–197, 1986
Kekow J, Hobusch G, Gross WL: Predominance of IgG1 subclass in the hypergammaglobulinemia observed in pre-AIDS ans AIDS. Cancer Detec Prevent 12: 211–216, 1988
Kaschka WP, Negele-Anetsberger J: IgG-Subklassenverteilung im Serum bei Patienten mit psychiatrischen Erkrankungen und gesunden Kontrollpersonen. In: Kaschka WP, Aschauer HN Thieme E (eds) Psychoimmunologie, 1990, pp 82–89
ASCO: Update of recommendations for the use of tumor markers in breast and colorectal cancer. J Clin Oncol 16: 793–795, 1998
Crombach G: Ñberlegungen zum rationalen Einsatz von Tumormarkern beim Mammakarzinom. Schweiz Rundsch Med Prax 87 (17): 589–594, 1998
Kobayashi T, Kawabuko T: Prospective investigation of tumor markers and risk assessment in early cancer screening. Cancer 73: 1946–1953, 1994
Bates S: Clinical application of serum tumor markers. Ann Intern Med 115: 623–638, 1991
Felsner P, Steinschifter W, Fischer M, Eferl R. Kenner L, Zatloukal K, Lahousen M, Liebmann P M, Schauenstein E, Schauenstein K: The tumor associated shift in immunoglobulin G1/G2 is expressed at the messenger RNA level of perpheral blood B lymphocytes of patients with gynecological malignancies. Cancer 88: 461–467, 2000
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Kronberger, L., Steinschifter, W., Weblacher, M. et al. Selective Decrease of Serum Immunoglobulin G1 as Marker for Early Stages of Invasive Breast Cancer. Breast Cancer Res Treat 64, 193–199 (2000). https://doi.org/10.1023/A:1006450205698
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DOI: https://doi.org/10.1023/A:1006450205698