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Steroid receptors in breast cancer: Sources of inter-laboratory variation in dextran-charcoal assays

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Summary

The presently recognized correlations between various clinical parameters and the concentrations of estrogen and progesterone receptors in breast cancer biopsies are largely based on receptor values obtained using the dextran-coated charcoal (DCC) method. This assay method is highly sensitive to slight changes in assay protocol, and differences in assay methodology may account for the wide variation in proportions of receptor positive patients reported by different centers.

A survey of various aspects of the assay method that may lead to reproducible, systematic differences in concentrations of receptor levels is presented; and methods of compensating for or correcting these potential differences are discussed. The following aspects are considered: a) constitution of biopsy tissue, b) method of tissue homogenization, c) adsorption of ligands to surfaces, d) inclusion of molybdate in the assay buffer, e) composition of the DCC slurry, and f) handling of samples for liquid scintillation counting.

Differences in methods used to homogenize tissue in Europe and the U.S.A. may account for differences observed in the correlation of DCC assay results to results obtained using the recently-introduced monoclonal ER-EIA technique.

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Authors and Affiliations

  1. Department of Clinical Physiology and Nuclear Medicine, The Finsen Institute, Strandboulevarden 49, 2100-DK, Copenhagen 0, Denmark

    Susan M. Thorpe

  2. Department of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark

    Susan M. Thorpe

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  1. Susan M. Thorpe
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Sponsored by the Danish Cancer Society

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Thorpe, S.M. Steroid receptors in breast cancer: Sources of inter-laboratory variation in dextran-charcoal assays. Breast Cancer Res Tr 9, 175–189 (1987). https://doi.org/10.1007/BF01806378

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